Abstract:Hyriopsis cumingii, which produces pearl oftbe highest quality, is a kind of freshwater species unique in China. The development of microsateUite markers of H. cumingii has not been reported. However, many microsateUite markers have been developed from Crassostrea gigas. The conservativeness of the side sequences of the microsatellite in species of close genetic relationship has already been discovered. In order to determine the applicability of micmsatellite primers developed from C. gigas to genomic analysis in H. cumingii, 32 polymorphic micmsatellite primers identified in the Pacific oyster were employed to amplify in the genome of mussel. The conditions of polymerase chain reaction(PCR) were optimized for the fidelity of DNA synthesis during PCR amplification. It was found that 19 loci failed to be amplified and 13 loci (about 41% ) amplified specific products successfully. Among 13 loci, 3 (Cgi-6, Cgi-27and Cgi-28) were monomorphic and 10 (about 31% ) (Cgi-l,Cgi-10,Cgi-18,Cgi-22,Cgi-24,Cgi- 25, Cgi-26, Cgi-29, Cgi-30 and Cgi-32) were polymorphic. The analysis of the genetic diversity showed the average heterozygosity of l0 microsateUites loci of H. cumingii ranged betwen 0. 125 and 0.693 and 7 loci (Cgi-10, Cgi-22, Cgi-24, Cgi-26, Cgi-29, Cgi- 30, Cgi-32 ) are high polymorphic(He 〉 0.500), whereas 3 loci (Cgi-1, Cgi-18 and Cgi-25 ) are low polymorphic(He 〈0.500). This study confirmed that 10 Pacific oyster pimers could be used for the analysis of the genetic diversity in H. cumingii. This result showed some of the microsateUite primers can be used for genetic analysis of mussel without high coat costing and time consuming. And it suggested that this method could be useful in genetic analysis of other species of mussel.