Mature eggs of Oreochromis aurea were obtained with artificial reproduction technology , and the hybrids were acquired with Siniperca chuatsi ( ♂) using artificial insemination . Genomic DNA of O. aurea ( ♀), S. chuatsi ( ♂) and their progeny were examined using RAPD method. Only 2 primers (S336 , OPZ06) of screened 11 random primers could be amplified into two specifically RAPD bands on progeny which were the same as those of S . chuatsi ( ♂) and did not exist in O. aurea ( ♀) , and other amplified bands on progeny were all from O. aurea ( ♀) . Genetic similarity index was 0. 858 between the progeny and O. aurea ( ♀) , while that was 0. 049 between the progeny and S . chuatsi ( ♂). This indicated that the genetic substance of progeny was mostly from Studies on hereditary relationship between Oreochromis aurea ( ♀), YANG Hong , XIA De2quan ,LIU Lei , WU Ting2ting ( Freshwater Fisheries Research Center , Chinese Academy of Fishery Sciences , Wuxi 214081 , Siniperca chuatsi ( ♂) and their offspring China) O. aurea ( ♀) . The primary function of sperm from S . chuatsi ( ♂) was stimulating development of eggs from O. aurea ( ♀) , at the same time little genetic substance of S . chuatsi ( ♂) appeared in the progeny , which laid the foundation for breeding of fine varieties having characters of O. aurea ( ♀) and S . chuatsi ( ♂).