Total RNAs were extracted from eyestalks of the shrimp Trachypenaeus curvirostris . Eyestalk complementary DNAs were obtained from these total RNAs by reverse transcription. Based on the amino acid sequence of the neuropeptide Pe-j SGP- . which has mol-t inhibit ing activity from Marsupenaeus jap onicus, two degenerate primers were designed. Polymerase Chain Reaction ( PCR) was performed using these primers and these eyestalk cDNAs. A specific cDNA was amplified under proper conditions. The specific cDNA was cloned into vector for sequencing . The sequencing results demonstrated that the specific cDNA from the shrimp T. curvirostris consisted of 213 base pairs. The alignment of the deduced amino acid sequence from eyestalk cDNA of the shrimp T . curvirostris with neuropeptides of CHH family from various crustaceans demonstrated that it had the highest identity and homology with most MIHs from various crustaceans. It suggested that the deduced amino acid sequence from eyestalk cDNA of the shrimp T . curvirostris was a part of MIH neuropeptide of T. curvirostris. The sequence corresponded to the amino acid sequence from 5 to 75 residues of MIH.