A recirculating system was designed for shrimp brood stock Penaeus vannamei culture indoor in the low salinity area of the river mouth in Shanghai. The natural seawater with salinity of 9 -12 was mixed with concentrated seawater with salinity of 100 -120 , which made the culture water salinity reach 28 -30. About 200 shrimp brood stock (female∶male was about 1∶1) were cultured in the recirculating system for two months , and the survival rate of the shrimp was about 90 %. Unilateral eyestalk ablation of female shrimp was done for stimulation of the ovary maturation. Three days after the operation the ovary development could be observed clearly , and one week later , a part of shrimp began to spawn , but the eggs were not fertilized. One month later fertilized eggs appeared and nauplius were obtained. The results showed that shrimp brood stock P. vannamei cultured in this recirculating system could live healthily and spawn successfully. The regularity of molting and growth in shrimp brood stock P. vanamei was investigated. Before ablation of eyestalk , the mean molt interval was about 14 ～ 15 days , and after that , the mean molt interval extends to about 20 days due to ovary development. Withmoltingthebodylengthofshrimpbroodstockincreasedcontinuously,whetherbeforeorafter ablation of eyestalk , but the mean daily increase of body length before ablation (female :0. 25mm , male :0. 20 mm) is greater than that after ablation (female :0. 17mm , male :0. 15) . The reasons of lower growth rate and longer molting interval were discussed.