Abstract:The morphology of the whole hemocyte and the variation of organelle and nucleus were observed by TEM in order to determine the composition of shrimp anticoagulant. A confirmed desirable anticoagulant is composedofNaCl 450mM,KCl 10mM,EDTA.Na210mM,HEPES 10mM (pH 7.45),which can maintain successfully the form of the whole hemocyte ,organelle and nucleus.