The hybrid cells produced by PEG2mediated cell fusion method with gill cells from flounder , Paralichthys olivaceus , and lymphocytes from shrimp , Penaeus chinensis , were cultured in mixed medium of MEM∶MPS (1∶1) . After 5 days culture and observation , it was found that the hybrid cells cultured in the mixed medium of MEM∶MPS (1∶1) grew into monolayer and with good growing and proliferating appearance. As the concentration of MPS in the mixed medium was raised gradually, ideal hybrid cells had been screened out. By now , the obtained hybrid cells have been subcultured for 12 generations and still had very good growing and dividing appearance. Hybridoma cells acquired obvious disease appearance such as ,the cell contracted to round , disattachmentanddeath,afterinoculationwithwhitespotsyndromevirus (WSSV) ofshrimpfor 10 days, and died almost completely at the 14th day after inoculation. After 0. 3 mL culture supernatants of the cells with obvious disease appearance added into a new monolayer of normal hybrid cells , the hybrid cells acquired obvious disease appearance 5 days later , while the control cells still with normal appearance. It can be concluded that the hybrid cells obtained in this study are propably the target cells of shrimp viruses.