雄核发育异育银鲫及其初步应用
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上海市水产研究所鱼类品种室

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上海市科学技术委员会资助项目( 鲫的应用研究) , 903213056 号。


The androgenetic allogynogenetic silver crucian carp and its priliminary application
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Fish Species Laboratory,Shanghai Fisheries Research Institute, 200433

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    摘要:

    用鲫( 2n= 100) 卵与异育银鲫( 3n= 156) 正常精子进行人工授精, 产生了部分的雄核发育异育 银鲫( 3n= 156) 。池塘中饲养的雄核发育异育银鲫性腺不仅能成熟, 而且也能生殖。它与父本异育银鲫回交 可以产生回交子一代( B1 ) 。雄核发育异育银鲫、回交子一代异育银鲫与父本异育银鲫非常相似。它们均具有 相同的染色体数目( 3n= 156) , 红细胞核的体积是鲫红细胞核体积的1. 6 倍。另一方面, 用随机扩增多态DNA ( RAPD) 标记检测了雌鲫、雄异育银鲫、雄核发育异育银鲫、回交子一代、雄红鲤的遗传多态性。RAPD- PCR 分子遗传标记结果表明与细胞学、细胞遗传学部分研究的结果相一致。因此, 雄核发育异育银鲫存在于鲫 ( .. ) 与3n= 156 异育银鲫( .. ) 杂交的部分F1 之中。

    Abstract:

    In this study part andtrogentic 3n= 156 allogynogentic silver crucian carp( 3n AASCC) using Carassins auratus eggs artif icially fertilizing with normal sperm from 3n= 156 allogynogenetic silver crucian carp ( 3n= 156 ASCC ) were produced . The 3n ASCC fed in pond can not only rearch sexual maturity , but be reproduced. Meanwhile, backcrossing females 3n AASCC ( F1 - similar to ASCC) to faternal parent 3n= 156 ASCC could produce f irst backcross generat ion ( B1 ASCC being very similar to faternal parent ASCC) . These have the same chromosomal numbers, e. g. 3n= 156, and volume of the erythr ocyte nuclei is about 1. 6 time whichis as big as that of C. auratus . On the other hand, genetic variat ions in females C. auratus , males 3n= 156 ASCC, 3n AASCC, backcross generation B1 and red common carp ( Cypinus carpio red variety) were detected using random amplifies polymorphic DNA(RAPD) markers. The result of molecular genetic markers indicated by the RAPD- PCR is consistent with that of the studies on cyto logy and cytogenetics ( chromosomes) . Therefore, androgenetic 3n= 156 ASCC exists in the part of F1 of C . auratus .. .. 3n= 156 ASCC .. .

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俞豪祥.雄核发育异育银鲫及其初步应用[J].水产学报,2000,24(1):

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  • 收稿日期:2014-04-17
  • 最后修改日期:2014-04-17
  • 录用日期:2014-04-17
  • 在线发布日期: 2014-04-17
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