The changes o f grow th, total lipid content and fat ty acid composit ion o f N . clost erium , N . p leacea and Pavlova sp . culture d at dif ferent temperatur e w ere investig ate d. As essentia l fa tty acid ( EFA) o f la rval o f shrimp, crab, bivalves and marine f ish, 20B5( n - 3) wa s pr ov ided by N . clost erium and N . pl eacea , 22B6( n- 3) by Pavkova sp . . Opt imum temperatur e fo r maximum l ipid and EFA pro ductio n tog ether w ith faster growth was 20 e fo r N . closterium a nd Pavlova sp . , 15 e fo r N . p al eacea.