应用ELISA方法快速检测IHNV—B病毒
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RAPID DETECTION OF INFECTIOUS HEMATOPOITIC NECROSIS VIRUS BENXI STRAIN(IHNV-B)WITH THE ENZYMELINKED IMMUNOSORBENT ASSAY(ELISA)
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    摘要:

    采用酶联免疫吸附试验(ELISA)快速诊断病毒性疾病,由于具有灵敏度高、特异性强和简便等优点,已在医学和兽医学上得到了广泛的应用。在鱼类病毒检测方面,Dixon,P.F.和Hill,B.J.用ELI-SA方法(1983)快速检测传染性胰脏坏死症病毒(IPNV)和(1984)几种鱼类弹状病毒,江育林等(1990)曾报道用此技术快速检测IPN病毒,1990年在我国本溪虹鳟鱼苗中分离出一株弹状病毒,暂称之为传染性造血器官坏死症病毒中国本溪株(IHNV-B),由于该病毒对虹鳟(rainbow trout)鱼苗危害极为严重,为预防和控制其蔓延,本文报道了应用ELISA方法对感染的鱼苗、鱼卵和细胞培养物中的IHNV-B病毒进行快速检测,可用目测或酶标检测仪来判定结果。

    Abstract:

    The short--snout icefish, Salangichthys (Neosalanx) jordani Wakiya Takahasi,is one of commercial fishes in Tai Hu Lake. Experiments on artifical fertilization ofthe fish were carried out in spring of 1981 and 1983.The ripe egg of the icefish is spherical, on the egg membrance there bear 8--13slender filaments radiating around the margin of micropyle, the filament are dichoto-mously divided directing to the opposite pole of the micropyle and connected each otherto make a network covering almost the entire surface of the egg. The eggs are strongsticky, the spawned eggs attach on the submerged plants and other matrix in thewater. The diameter of swollen egg is 0.59--0.73mm, average 0.64mm. The embryohatched out 149 hours after the fertlization, 865 prelarvae were obtained in the experi-ment. The protection of spawning ground of the icefish, especially the submergedplants is proposed.

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赵志壮,牛鲁祺,刘长明.应用ELISA方法快速检测IHNV—B病毒[J].水产学报,1993,17(1):60~63

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  • 收稿日期:1992-07-01
  • 最后修改日期:1992-10-01
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  • 在线发布日期: 2014-10-23
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