Abstract:Feeding experiments were conducted to determine the daily requi-rement of protein, fat, mineral and general energy for juvenile turtle. The L9(34)table of orthogonal design method was employed to formulate nine diet combinatios.The experiments were conducted in 65 x 50 x 50cm aquaria under the following con-ditions,(1) water depth 20cm, water volume 65 l. (2) water flaw maintained at 2 .1-3.0 l/hr. (3) water temperature averaged 28 ±20℃, and (4) dissolved oxygen 0.12-1.94 mg/l,pH6.9---7.1 and NH4+-N 0.1---1.8 mg/l. The weight gain rate,feed conversion and protein efficiency ratio (PER) were used as the index to eva-luate the feeding effect. The results indicate that under the circumstances of the experiments the protein and general energy are major elements that affect the growth of the juvenile turtle. The optimum general energy of the most satisfactory diet is 320-340 kcal/100g feeds, pxotein 46.6%,fat 3--5%, mineral 2-3%, rar-bohydrate 21--28%. 100g juvenile turtle need 0.648g protein, 4.5-4.7kcal gene-ral energy,0.042---0.070g fat, 0.32--0.43g carbohydrate,mineral 2--3%and fiber 20----22%. The optimum calorie-protein ratio (c/p value) in formulated diet for ju-venile turtle is 7.00---7 . 55.