In manured fish ponds, the conversion of manure-feeds-fish undergoes a complicated process of pond dynamics. In order to well understanding andmanipulation of this process so as to improve farming productivity, we introducedcarbon isotype analysis into the experiment. We accurately measured Delta C in organic matters such as fish body, fish feede and manure. The orientation of carbonwas traced by means of mass spectrometer and the interactions of fish growth andcomplicated food web texture in manured pond were analyzed and studied. The resultsshowed that the species of filtering and omnivorous food habits in manured pondobtain carbon from both autotrophic and heterotrophic resources. Obviously, it is ofpractical significance in organising aquanultural activities and making clear the ponddynamies.