By using the technic of tissue culture we have successfully established a strain ofcomplex of epitheial-like and fibroblast-like cells from the snout tissue of grass carp(Ctenopharyngodon idellus), and it is denoted as strain ZC--7901. The medium used inthe cultivation is"199" supplemented with 15% fetal bovine serum containing a mode-rate amount of antibiotics. The incubation temperature is at 25--28℃. A substrain ofsingle epitheial-like cells is also obtained from ZC--7901 cells of 11th generation, whichis named substrain ZC--7901S_1. At Present both the strain and substrain have been maintained in proliferationfor over 22 months through 120 and 90 generations respectively, and the cells ofthem are able to reproduce with approximately 1.5×10~5 cells per millilitre in con-centration. About 4 days, a monolayer cells can be found clearly in the culture. After36 hours, the index of mitotic division of the ZC-7901 is 36.5‰, while that of theZC-7901S_1 is 32.3‰; and then at 156 hours after propagation the former is gradullydecreased to 12.3‰ and the latter to 14.3‰. The chromosome number of each cell inthese two strains is 2n = 48.