采用高压液相色谱测定降血压肽的血管紧张素转化酶抑制活性，对碱性蛋白酶水解草鱼蛋白制备鱼降压肽进行研究。结果表明，碱性蛋白酶水解草鱼蛋白制备鱼降压肽的较佳工艺条件为：pH9.0、温度50℃、酶与底物比48AU.kg-1、水解度34.52%。鱼降压肽成分分析结果显示，鱼降压肽中可溶性氮含量为81.26%，肽含量为72.81%，脂肪为0.12%，水分为3.54%，灰分为9.47%。鱼降压肽的血管紧张素转化酶抑制活性为70.35%；体积排阻色谱测定鱼降压肽的相对分子质量在124～10581之间，主要集中在124～1062之间；溶解度的测定结果表明，鱼降压肽在pH3.0～11.0时溶解度为96.0%左右，可广泛应用于食品中。 关键词：草鱼； 降压肽；碱性蛋白酶；高压液相色谱；相对分子质量；体积排阻色谱

China is the rich country of fishery on fresh water, the yearly output of fresh water fish is 19.188 million tons, approximately occupy 40% of the total fish output. The main kinds of fish are the silver carp, variegated carp and grass carp. The processing proportion reaches above 75% in developed country, and there is only 30% in our country at present. The fresh water fish are sold primarily fresh with the low price, which has seriously affected the sustainable development of fresh water fishery. Not only have antihypertensive peptides from protein of fresh water fish the advantage of protein, but also they have the character of acid and hot stability as well as the better solubility and unique antihypertensive function, it is easier to digest and absorption than the protein and its amino acid. So It will be the future for deeply processing fresh water fish that fish protein was utilized to prepare for antihypertensive peptides by biological technology. In the paper grass carp protein was hydrolyzed by alcalase and angiotensin Ⅰ-converting enzyme inhibitory activities was assayed by high-performance liquid chromatography for preparing fish antihypertensive peptides. The results indidated that appropriate hydrolysis conditions for alcalase were pH9.0, 50℃, ratio of alcalase to grass carp protein with 48 AU.kg-1, DH34.52%. The analyzing results of components showed that fish antihypertensive peptides made at the same hydrolysis conditions contained 81.26% of soluble N, 72.81% of peptides, 0.12% of fat, 3.54% of water and 9.47% of ash. Angiotensin Ⅰ-converting enzyme inhibitory activities of fish antihypertensive peptides was 70.35%; Its relative molecular weight assayed by size exclusion chromatography distributed from 124 to 10581, and mainly from 124 to 1062. Solubility of antihypertensive peptides from grass carp protein was analyzed during pH3 and pH11, the result showed that its solubility with 96.0% around was steady, and fish antihypertensive peptides could be extensively used in the food. Key words: grass carp; antihypertensive peptides; alcalase; high-performance liquid chromatography; molecular weight; size exclusion chromatography

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