Home > Archive>Volume 42, Issue 7, 2018 >1124-1131. DOI:10.11964/jfc.20161210661
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Fermentation optimization of a strain Bacillus methylotrophilus WM-1 against Aeromonas hydrophila
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College of Animal Science and Technology, Southwest University, Chongqing 400715, China,,,College of Animal Science and Technology, Southwest University,

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    Abstract:

    To investigate and obtain the best medium formula and fermentation conditions of an antagonistic strain WM-1 against Aeromonas hydrophila, we used A. hydrophila as the index bacteria of the antibacterial. The activity of strain WM-1 was measured by Oxford cup test. Single factor experiments and orthogonal experiments were employed to optimize the liquid medium compositions and fermentation conditions of WM-1. The data showed that the optimum medium included 1.0% soluble starch, 1.5% beef extract, 0.05% K2HPO4·3H2O and 0.07% MgSO4·7H2O; and the optimal fermentation conditions were initial pH 7.5, culture temperature 30℃, fermentation time 48 h, medium volumes 20%, rotating speed 210 r/min. Under the optimum conditions, the inhibition zone of WM-1 reached (22.5±0.50) mm, which was 40.6% higher than that under non-optimized conditions. These results provide a theoretical basis for the biocontrol of A. hydrophila.

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ZHANG Jiaojiao, MA Fuping, LI Yanhong, WU Zhengli, YAO Weizhi. Fermentation optimization of a strain Bacillus methylotrophilus WM-1 against Aeromonas hydrophila[J]. Journal of Fisheries of China,2018,42(7):1124~1131

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History
  • Received:December 26,2016
  • Revised:May 04,2017
  • Adopted:May 14,2017
  • Online: June 27,2018
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