Based on the morphological measure ment and biochemical analysis,this study was conducted to compare the morphology,hepatosomatic index (HSI) and biochemical composition between wild-caught (WC) and pond-reared (PR) juvenile Eriocheir sinensis.The results showed that:the significant differences were found on the morphology of WC and PR.There were four of dominated principal component (i.e.PC1-PC4) for both females and males.PC1 contributed more significantly to the principal component analysis than the other PC,and PC1 of both gender consist of 16 and 19 main variables,respectively.Among the 36 morphological indices,there were 25 and 22 indices with significant differences between WC and PR,for males and females.Four to five indices were further selected to establish discriminant equation,and those equations had more than 95% of accuracy for distinguishing WC and PR.WC had the higher HSI,tissue total lipids,muscular 16:0,18:1n9 and 20:4n6 levels,but the lower moisture contents,muscular 18:0,20:5n3 and 22:6n3 levels than PR.Therefore,the effective identification methods are established based on their morphology and biochemical composition between WC and PR.