Effect of dietary carbohydrate-to-lipid ratio on growth, blood biochemical indices,hepatic metabolic enzymes and <em>PEPCK</em> gene expression of juvenile obscure puffer(<em>Takifugu obscurus</em>)

doi:10.3724/SP.J.1231.2014.49175

Home > Archive>Volume 38, Issue 8, 2014 >1149-1158. DOI:10.3724/SP.J.1231.2014.49175

Effect of dietary carbohydrate-to-lipid ratio on growth, blood biochemical indices,hepatic metabolic enzymes and PEPCK gene expression of juvenile obscure puffer(Takifugu obscurus)
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                        10.3724/SP.J.1231.2014.49175
                    
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                        LIU XiangheLIU Xianghe
Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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YE ChaoxiaYE Chaoxia
Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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SHEN BiduanSHEN Biduan
Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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WANG ChunyanWANG Chunyan
Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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PENG JianPENG Jian
Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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WANG AnliWANG Anli
Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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Affiliation:Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University,Key Laboratory of Ecology and Environment Science of Guangdong Higher Education Institutes,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,College of Life Science,South China Normal University
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Abstract:

This experiment was conducted to evaluate the effects of dietary carbohydrate-to-lipid ratio on growth performance, feed utilization, biochemical indices and phosphoenolpyruvate carboxykinase(PEPCK)gene expression in juvenile obscure puffer, Takifugu obscurus.Six isonitrogenous and isoenergetic diets were formulated with dietary CHO:L ratios of 4.29, 2.79, 1.86, 1.19, 0.73 and 0.42, respectively.Fish(initial weight 11.2±0.5 g)were stocked in triplicate tanks(25 fish per tank)and fed to satiation during a period of 60 days.The results indicated that best specific growth rate, protein efficiency ratio and feed efficiency were found in fish fed diet with CHO:L of 1.86, which were significantly higher than those of fish fed diets with 0.42, 0.73 and 4.29(P<0.05).Plasma triglyceride and liver lipid content increased significantly as dietary CHO:L ratio decreasing(P<0.05), while plasma glucose and hepatic glycogen content showed a contrary tendency.Activities of lipase, fatty acid synthase, amylase and pyruvate kinase in liver were significantly affected by dietary CHO:L ratio(P<0.05).Fish fed diets with CHO:L of 0.42 and 0.73 had significantly higher PEPCK activity and mRNA relative expression than those fed with the other diets(P<0.05).However, survival and plasma total cholesterol content were unaffected by dietary CHO:L ratio.The optimal range of CHO:L within 2.01-2.16 is suitable for juvenile obscure puffer based on second-order polynomial regression analysis of SGR, PER and FE against dietary CHO:L ratio, respectively.

Key words:Takifugu obscurus;carbohydrate-to-lipid ratio;biochemical index;PEPCK gene

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LIU Xianghe, YE Chaoxia, SHEN Biduan, WANG Chunyan, PENG Jian, WANG Anli. Effect of dietary carbohydrate-to-lipid ratio on growth, blood biochemical indices, hepatic metabolic enzymes and PEPCK gene expression of juvenile obscure puffer(Takifugu obscurus)[J]. Journal of Fisheries of China,2014,38(8):1149~1158

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Received:March 12,2014
Revised:May 12,2014
Adopted:June 20,2014
Online: August 21,2014
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