Molecular cloning and expression analysis of the IgM heavy chain gene in largefin longbarbel catfish(Mystus macropterus)

doi:10.3724/SP.J.1231

Home > Archive>Volume 35, Issue 11, 2011 >1684-1693. DOI:10.3724/SP.J.1231

Molecular cloning and expression analysis of the IgM heavy chain gene in largefin longbarbel catfish(Mystus macropterus)
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                        10.3724/SP.J.1231
                    
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                        [cstr]
                    
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Affiliation:Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University,Hydrobiology Institute of Jinan University,Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University,Key Laboratory of Aquatic Science of Chongqing, Key laboratory Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), School of Life Science, Southwest University,Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University,Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University
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Abstract:

Full cDNA sequence of a secretory IgM(sIgM)gene in largefin longbarbel catfish(Mystus macropterus Bleeker)was cloned using RACE-PCR and RT-PCR methods.It has 1 992 nucleotides,including 5′UTR of 53 nucleotides,3′UTR of 226 nucleotides and an open reading frame with 1 713 nucleotides encoding a 570 amino acid peptide.The deduced amino acid sequence in M.macropterus contains 4 constant regions(CH)and a variable domain(VH)which consists of 4 frame regions (FRs)and 3 complementary determining regions(CDRs).The IgM comparison in seven teleost species showed that IgM in M. macropterus shared the highest identity(54.3%)with that in Ictalurus punctatus, and the lowest identity(24.6%)with that in Epinephelus coioides, and that the conserved cysteines and tryptophans existed in all fishes involved.Phylogenetic tree based on some teleost Ig heavy chain amino acids suggested IgM in M.macropterus was clustered closely with that of I.punctatus. Fluorescent real-time PCR showed that IgM mRNA expression of M. macropterus was mainly detected in blood cells,spleen,kidney and head kidney,and increased significantly in blood cells,spleen,head kidney between day 1 and day 10 after injection of Aeromonas hydrophila.

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LI Chuntao, ZHANG Qizhong, YANG Yingying, ZHU Chengke, LI Chao, CHEN Xia. Molecular cloning and expression analysis of the IgM heavy chain gene in largefin longbarbel catfish(Mystus macropterus)[J]. Journal of Fisheries of China,2011,35(11):1684~1693

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Received:June 03,2011
Revised:July 10,2011
Adopted:September 14,2011
Online: November 15,2011
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