Abstract:To research the sensory physiology functions of peculiar sense organs on the abocular side of Cynoglossus semilaevis, both mechanical and chemical stimulations of the peculiar sense organs were investigated by using electrophysiological techniques.The mechanical stimulations can be demonstrated by evoking discharges from the nerves of peculiar sense organs after being touched with glass hooks lightly,which were recorded by Biomedical Multichannels Data Acquisition System.The results of small and big mechanical stimulations showed that stimulatory effects were significantly affected by the strength of mechanical stimulations; While using six kinds of L-amino acids,dimethyl sulphoxide and clam worm as chemical subjects,chemical stimulations of the dermal surface structures were also checked by the evoking discharges recorded by Biomedical Multichannels Data Acquisition System,and the frequencies and scopes of evoking discharges from the nerves of peculiar sense organs were raised as the concentrations of chemical subjects increasing.In comparison with the relations about natural responses and concentrations of chemical subjects,we knew that the order of the strength of chemical subjects was:clam worm >L-Arg>L-Met>L-Lys>L-His>dimethyl sulphoxide>L-Gly>L-Ala.The results indicated that peculiar sense organs on the abocular side have dual sensory function to both mechanical and chemical sensitivity.Since C.semilaevis depend on sensory organs to find food,it is probable that the dermal surface structures may be relative to feeding activities,and have an active effect on feeding of tongue sole.This study develops a research about peculiar sense organs on the abocular side of C.semilaevis using electrophysiological techniques,which may serve as a valuable resource for further ultrastructure and behavioral science research of C.semilaevis.