Microsatellites combined with bulked segregation analysis(BSA)were used to screen the gender differences of 96 individuals(48 for male and female each)from a family of Channa argus.Two gene pools were constructed using 24 individuals for male and female each separately,gene pools were used to scan using totally 140 pairs of microsatellite primers and specific DNA fragments of female gene pool were amplified by 3 pairs of primers(HLJWL17,HLJWL59,HLJWL70).Then,the specific DNA fragments amplified by the three pairs of primers were verified for the first round in the 48 individuals constructing the gene pools,showing that the specific bands of HLJWL17 and HLJWL70 in gene pools only appeared in few individuals,while HLJWL59 was successfully amplified in females.And in the second round verification,the same results were obtained in the remaining 48 individuals with the HLJWL59.The amplified fragments by primer HLJWL59 of eight different alleles in females were cloned and sequenced.Sequence alignment of the eight individuals using Vector 8.0 multiple confirmed that the bands of each individual are the same sequence,with the sequence length of 243 bp.The repeat units were TGC,and 51% of GC.Compared by the BLASTn,homologous sequences were not found in the GenBank database.According to the statistics,the probability of different bands that appeared in the female C.argus was 62.5%,that is,the correct classification rate of the marker for female C.argus was 81.25%.We believe that this microsatellite loci may be associated with C.argus female gender under certain conditions.This study may serve as a basis for culturing pure male hybrid using female-specific DNA fragments in C.argus.