Using artificial spawning and dry insemination,experiments on interspecific hybridization within females of Spinibarbus sinensis and Spinibarbus denticulatus and males of S.sinensis and S.denticulatus and Spinibarbus caldwelli of Spinibarbus were conducted.Comparisions of egg membrane diameter,fertilization rate,hatching rate,hatching time,abnormality rate,early larvae morphological characters and survival rate were made at temperature of (27±0.5)℃.Meanwhile the embryonic development and abnormal phenomena of hybrid were observed.The results showed that there was no distinct difference on egg membrane diameter between the crossbreeding and the oosperm of the female parent of S.sinensis(P<0.05).There was no distinct difference on egg membrane diameter between the crossbreeding and the oosperm of the female parent of S.denticulatus at multicellular stage,but there was distinct difference on egg membrane diameter between the hybrid of S.denticulatus ♀×S.sinensis ♂ and the oosperm of the female parent of S.denticulatus at otolithes appearance stage(P<0.05).The fertilization rate and hatching time of hybrid were not significantly different,but the abnormal rates were significantly higher compared to their mother’s(P<0.05).The hatching rates of crossbreeding of S.sinensis were high and not significantly different compared to their mother’s(P<0.05).The hatching rate of S.denticulatus ♀×S.sinensis ♂ was not distinctly different,but the hatching rate of S.denticulatus♀ ×S.caldwelli ♂was significantly lower compared to their mother’s(P<0.05).There was high death rate stage in the early larvae development of crossbreeding of S.sinensis,and the survival rates were significantly lower than their mother’s(P<0.05).There were characteristics partly similar to the female parent or male parent for hybrids of crossbreeding of S.sinensis and S.denticulatus ♀×S.caldwelli ♂.It showed preliminarily that it was feasible for the interspecific hybridization of three species of Spinibarbus.