In order to assay the effects of different bubbling modes on microalgae during cultivation,the growth,biochemical composition and utilization on N and P from medium for Dunaliella salina treated with three bubbling modes(shaking flask manually,continuous aeration and CO₂ bubbling)respectively were investigated in this paper.The results showed that the biomass(1.62×10⁷ind/mL)of D.salina treated with continuous aeration was significantly higher than those of the other groups after 5 days. D.salina mainly used the nitrogen stored in cell for growth and reproduction in the first 3 days.Then N in the culture medium was dramatically absorbed by D.salina cell.While the cell of D.salina could rapidly absorb P from the culture medium after inoculation and store P in the cell for the following growth and reproduction.After first three cultured days,N content in the culture medium treated with continuous aeration was significantly lower than those of the other groups.The bubbling mode did not affect the cell of D.salina to absorb P from the culture medium significantly.The contents of total lipid,crude protein and amino acid of D.salina treated with continuous aeration were significantly lower than those of the other groups.The content of PUFAs(polyunsaturated fatty acids)of the cell treated with continuous aeration was significantly higher than the other groups.It is therefore suggested that the bubbling modes affect growth as well as cell composition of the microalgae D.salina.The growth performance and fatty acid nutritional value of the cell treated with continuous aeration improve while the amino acid nutritional value of the same cell decreases.