The Zhikong scallop Chlamys farreri plays an important role in the shellfish breeding in the north of China and the gynogenesis in this scallop has developed rapidly in recent years. In this study, the meiotic division of the eggs of C. farreri was activated by ultraviolet (UV)irradiated heterogenous sperms of Pacific oyster Crassostrea gigas and the gynogenetic diploids were induced by blocking extrusion of the second polar body from fertilized eggs with 6dimethylaminopurine (6-DMAP). To assess homogeneity and isogenedity between the progeny and their parents, microsatellite technique were used. Then 3 pairs of common microsatellite primers to be used for genetic analysis of offspring were screened from 21 pairs of microsatellite primers among which 11 pairs were indentified in C. farreri and 10 pairs in C. gigas by optimizing the condition of polymerase chain reaction(PCR). Under the optimal condition, PCR was performed for analyzing the genetic character of the gynogenetic offspring. It was found that the 3 loci could amplify clear fragments in these offspring. The results of genetic analysis showed that the gene of offspring without gene contamination of male parent C. gigas was completely inherited from female parent C. farreri. Microsatellite locus and centromere recombined at the 3 polymorphic loci and the recombination rates were 40%, 55%, and 35%, respectively. This study confirmed that it was feasible to achieve diploid gynogenetic progeny of C. farreri by heterologous sperm’s inducement while it was difficult to get an absolute homozygotic population once in manner of suppression of the meiosisⅡ, because there was high heterozygosity in the first meiogynogenetic generation owing to crossover between the homologous chromosome during the meiosisI.