Viral nervrou necrosis virus (VNNV) causes the pathogenic effects in the nerve tissues of many marine fish, which caused great economic loss in mariculture of many countries. In this study, the capsid protein (CP) gene of VNNV 0603 Strain was amplified successfully by reverse-transcription polymerase chain reaction (RT-PCR), and inserted into plasmid pFastBacⅠof the Baculovirus expression system Bac-To-Bac to develop plasmid pFastBac-cp. Through homologous recombination between pFastBac-cp and Bacmid which was the modified genome of Autogragha california nuclear polyhedrosis virus (AcNPV), the recombinant shuttle vector named Bacmid-cp was obtained in E. coli DH10Bac. After tranfection with Cellfectin Reagent, recombinant Baculovirus (AcNPV-cp) was formed in Sf9 cells. The specific 37kD protein band and the hybridizing reaction between expressed protein and positive serum for VNNV were showed by analysis of SDS-PAGE and Western blotting, which indicated that VNNV CP protein had been expressed in insect cells Sf9 with immunological activity. The self-assembled virus-like particles (VLPs) were observed in the negative staining CP crude extract and in the ultra thin section of infected Sf9 cells with electron microscopy, which were similar to VNNV whole virion, and became a crystal array in cytoplasm. On the base of present study, the particle vaccine of VNNV would be developed and put into practice in the near future.