Bacterial flora and identification of dominated spoilage organisms on cultured Pseudosciaena crocea at the end of shelf life during storage at 0 ℃ and 5 ℃ were qualitatively and quantitatively investigated, based on the colony and cellular morphology, biochemical and physiological characteristics as well as wholecell fatty acid composition, and applied with Sensititre，BioFosun，MIDI bacteria identification systems. The dominated bacteria of Shewanella putrefaciens were 75.5% and 59.6% at the end of shelf life at 0 ℃ and 5 ℃,and its colonies on nutrition agar and trypticase soy broth agar were global apophysis, trim side, smooth clarity, achromatism to pink. The cells were gram negative rod［(0.6-0.9) μm×(1.5-3.3) μm］ and motile by means of polar flagellum, the optimum growth occurred at 25-35 ℃ and pH 7-9. Strains were positive for oxidation，H2S，TMAO，gelatin and Tween 40. Strains utilized NacetylD-glucosamine, ect. The dominant fatty acids were 16∶1ω8c, iso15∶0，17∶1ω8c，16∶0, mean contents were 21.31%，13.63%，9. 82%，9.76%， respectively. Results from tested strains were compared with database values in the bacterial identification systems, indicating the dominated spoilage organisms were Shewanella putrefaciens.