Hematological parameters of different age groups of cultured Acipenser schrenckii
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East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China

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    Abstract:

    Using hematology analyzer, hematological parameters of 5 age groups ( 1 - 5 years old) of cultured Amur sturgeon, Acipenser schrenckii, were studied. Blood samples were collected using 5 mL medical injectors from the artery or vein near the anal fin of the fish, and the samples were put into tubes with anticoagulant for examination. For each age group, 5 individuals of fish were sampled. Before blood sampling, fish was immobilized using MS-222 at the concentration of 200 mg? L^-1. Examination data were analyzed using software STATISTICA version 6.0 ( Statsoft, Inc. ), and using one-way ANOVA to test the significance of group differences, using LSD for multi comparison, and using GRM for the correlative analysis. The examination results showed that red blood cell (RBC), haematocrit (HCT), hemoglobin ( HGB ), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell count (WBC) increased with the age. WBC and HCT of 5 years old group were significantly higher than that of 1 - 4 year-old groups ( P 〈 0.05 ), however, there was no significant difference of HGB, MCH and MCHC among all groups. Among 1 - 4 year-old groups, there was no significant difference in mean corpuscular volume (MCV), platelet (PLT) count and hemoglobin distribution width (HDW), but the MCV and PLT of 5 years old group increased significantly and HDW reduced significantly. RDW did not change much with the age, but mean platelet volume (MPV) slightly reduced with the age. For the change of differential leukocyte count (DLC), the proportional order of the number of different kinds of cells in the leucocyte indicated lymphocyte (Lym) 〉 basophil (Baso) 〉 neutrophil (Neut) 〉 monocyte (Mon). With the increase of age and Lym reduced, however, Baso and Mono increased. Comparison with other fish species, HCT and RBC of Amur sturgeon were lower than that of most freshwater fish species. MCV of culaued Amur sturgeon was lower than those of wild ones, but MCHC of culaued Amur sturgeon was much higher than that of wild ones. This result is similar to the test of rainbow trout and tilapia. Lym number of culaued Amur sturgeon was high, so was that of the most fish species. Similarly, Baso number of culaued Amur sturgeon was high also, just below the Lym number. This is quite different from that of most freshwater fish species. MCV, PLT and HDW of 5 years old culaued Amur sturgeon were significantly different from those of 1 - 4 year-old groups. This result may imply that these parameters may be related to the age of maturation because 5 years old culaued Amur sturgeon reached maturity.

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ZHANG Long-zhen. Hematological parameters of different age groups of cultured Acipenser schrenckii[J]. Journal of Fisheries of China,2006,30(1):

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History
  • Received:March 09,2014
  • Revised:March 09,2014
  • Adopted:March 09,2014
  • Online: March 09,2014
  • Published: