In order to study the potential functions of DEAD-box family genes p68 and p110 in germ cell proliferation and diffemtiation, using in situ hybridization on tissue sections with DIG labeled antisense RNA probe, we detected the location of goldfish （Carassius auratus） DEAD-box family genes p68N and p110 RNA during gametogenesis. The results are as follows： during goldfish oogenesis, the expression of p68 RNA was observed in oocytes at all stages, and its expression was higher in stage Ⅰ , Ⅱ oocytes than in stage Ⅲ, Ⅳ oocytes. The expression of p110 RNA could not be found in stage Ⅰ oocytes and strong signals could be detected in stage Ⅱ oocytes, but the signals became much weaker in stage Ⅲ, Ⅳ oocytes. During goldfish spermatogenesis, the expression of p68 RNA could only be detected in spermatogonia and primary spermatocytes. The expression of p110 RNA was visible throughout spermatogenesis, and its expression was much higher in spermatogonia and primary spermatocytes than in spermatids. Based on the active expression of p68 and p110 during goldfish gametogenesis, we speculated that they may play potentially important roles in germ cell development of both sexes.