This paper reported the effects of disposal methods of sample on determination of digestive enzyme of tilapia and silver crucian carp. The results showed that activities of digestive enzyme in intestine tissue homogenized for 1 min and 2 min ( 10000 r#min- 1) respectively were not remarkably different, and so did that frozen and melted once. By centrifugation, the activities of digestive enzyme in superstratum of intestine tissue homogenized were 2. 8%- 7. 4% lower than those of initial intestine tissue homogenized. At 4 e , the activities of digestive enzyme in intestine tissue homogenized were the most stable in the phase of 3- 9 h and 9- 18 h for protease and amylase of silver crucian carp respectively after homogenization, and so did that in the phase of 6- 9 h for both protease and amylase of tilapia, in the other phase, the activities of digestive enzyme in intestine tissue homogenized decreased at most by 9. 0% - 18. 2% and 11. 5% - 44. 4% for protease and amylase respectively.