1982day feeding trials were conducted to determine the reciprocal physiological effects on Chinese mitten2handed crabs Eriocheir sinensis female broodstock by feeding with four experiment diets and one control group diet for: diet 1 supplementing HUFA and vitamin E, C; diet 2 supplementing only vitamin E and C; diet 3 supplementing neither HUFA nor vitamin E and C; diet 4 supplementing only HUFA , diet 5 (control group diet) was fresh clam. The results showed that vitamin E, vitamin C and HUFA were essential components in broodstock diets. Fecundity and hatchability of eggs from broodstock crabs fed with diet 1 in which HUFA and vitamin E, C were supplemented were 3790 egg cell·g 1 and 86. 17 % respectively , were the very significantly higher than those of broodstock crabs fed with HUFA2deficient diet like diet 2 and 3 (2317egg cell·g-1, 36.06 % and 2129egg cell ·g-1, 29. 18 %) ( P < 0. 01) , and significantly higher than those of and fecundity and hatchability of eggs (3050 egg cell·g 1 and 25. 73 % in diet 4 treatment) from the crabs fed with diet 4 were also lower than those of control group (3010 egg cell·g 1 and 71. 12 % respectively) ( P < 0. 05) . When the broodstock crabs were fed with vitamin E and C2deficient diets like diet 3 and 4 , lipids of crabs were peroxidized with decreasing SOD activity and increasing MDA content. Meanwhile , the effect of total lipid content , fatty acid composition and vitamin E and C concentrations of experimental diets on those of corresponding eggs , and the lipid peroxidation in eggs are discussed. The results of this study indicate both HUFA and vitamin E , C are indispensable and cooperative to egg developing of crab and must be supplemented in the broodstock crab diet.