Monoclonal antibodys (Mab) raised against Vibrio alginolyticus strains (1. 1587) by that primed spleen cellsfusedwithSP2/0myelomacellsusingpolyethylingglycol. Therateoffusionwas 45.8%andtherateof positiveinfusionwas 77.4%byELISA. Mabs, AC1-C9,AC1-C11 ,BB4-D4,AD1-A3,AD1-F3,AD2E7 ,AD2 -F7 , were harvested using usable hybridomas clone twice by limiting dilution. Ascites with Mabs against Vibrio alginolyticus were harvested by injecting BALB/ C mice. The titer of the ascites was 1 : 1000. In comparison , Mab AD1 -F3 reacted with only four Vibrio alginolyticus strains . No cross2reactions were observed among thirteen non2Vibrio alginolyticus. Using this Mab ascites , an indirect Enzyme2Linked Immunosorbent Assay (ELISA) for rapid diagnosis of Vibrio alginolyticus has been developed. This method can detect 1 ×104 bacteria per ml with in 6 -7 h. After the method was optimized , 11 samples were detected for Vibrio alginolyticus.