A new goby, Gymnogobius zhoushanensis, is described from four specimens that collected from a stream of Zhoushan Island (30°02’N, 122°03’E) of Zhejiang Province in China. This new species is similar to the species Gymnogobius transversefasciatus (Wu & Zhou, 1990) in having four sensory canal pores (C，D，F，G) on head and four longitudinal sensory papilla lines on cheek, but defers in many characters. The former maxillary only reach to beneath the anterior edge of eye (vs. maxillary reach to beneath the posterior edge of eye): eye 4.3 to 5.5 in head (vs. 5.8 to 7.2)；the second dorsal fin with 11 to 12 rays (vs. 9 to 10)；longitudinal scales 54 to 56 (vs. 79 to 81)；transverse scales 11 to 12 (vs. 20 to 22)；predorsal scales 12 to 15 (vs. 17 to 21)；head with a lot of small black spots (vs. without black spots). Key words: Gobiidae；Gymnogobius；new species；Zhoushan