文章摘要
卵黄抗体对凡纳滨对虾生长、免疫及抗VpAHPND感染的影响
The effect of IgY on the growth, immunity and resistance to VpAHPND infection of Litopenaeus vannamei
投稿时间:2021-02-03  修订日期:2021-03-12
DOI:
中文关键词: 卵黄抗体  凡纳滨对虾  生长性能  急性肝胰腺坏死病  免疫应答  基因表达
英文关键词: Immunoglobulin of yolk (IgY)  Litopenaeus vannamei  growth performance  immune response  gene expression  acute hepatopancreatic necrosis disease (AHPND)
基金项目:农业农村部农业国际合作交流项目;国家自然科学基金 (31802342);中国水产科学研究院基本科研业务费 (2020TD39)
作者单位邮编
王仁宝 上海海洋大学水产与生命学院 201306
史成银 中国水产科学研究院黄海水产研究所 266071
宋晓玲 中国水产科学研究院黄海水产研究所 
王一婷 中国水产科学研究院黄海水产研究所 
张惠芬 中国水产科学研究院黄海水产研究所 
任宁欣 上海海洋大学水产与生命学院 
谢国驷 中国水产科学研究院黄海水产研究所 
张庆利 中国水产科学研究院黄海水产研究所 
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中文摘要:
      为探讨特异性卵黄抗体抗致急性肝胰腺坏死病副溶血弧菌 (Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease, VpAHPND) 感染的效果及其机制,防控对虾急性肝胰腺坏死病 (Acute hepatopancreatic necrosis disease, AHPND),本研究以添加不同剂量VpAHPND卵黄抗体制剂 (0、0.2%和0.5%) 的饲料投喂凡纳滨对虾幼虾,测定对虾的生长率和存活率、对虾肝胰腺免疫酶活力和免疫基因相对表达水平,通过浸浴感染实验测定免疫对虾抗VpAHPND感染的能力。生长实验结果显示,免疫28 d后,免疫组与未添加卵黄抗体制剂的对照组对虾在平均生长率、特定生长率和存活率方面均无显著性差异。免疫功能实验结果显示,免疫14 d后,与对照组相比,0.2%免疫组对虾肝胰腺的酚氧化酶 (PO)、超氧化物歧化酶 (SOD)、溶菌酶 (LZM) 活力显著升高,抗菌肽 (Crustin) 基因的相对表达水平也显著升高,而β-1,3-葡聚糖结合蛋白-脂蛋白 (β-GBP-HDL) 基因的相对表达水平则显著降低。浸浴感染实验结果显示,0.2%免疫组对虾的存活率显著高于对照组;0.2% VpAHPND卵黄抗体制剂对凡纳滨对虾的相对免疫保护率为63.77%。研究表明,口服卵黄抗体不会对凡纳滨对虾的生长和存活产生不良影响,0.2%的VpAHPND卵黄抗体制剂通过提升凡纳滨对虾的PO、SOD、LZM活力和Crustin基因表达水平,增强凡纳滨对虾的免疫功能,从而提高对虾抗VpAHPND感染的能力,具有很强的应用潜力。本研究为使用特异性卵黄抗体防控AHPND提供了依据,也为其作用机制研究提供了参考。
英文摘要:
      Acute hepatopancreatic necrosis disease (AHPND) is the major bacterial disease of cultured shrimps, mainly caused by Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (VpAHPND). The aim of this study was to explore the anti-AHPND effect and its mechanism of immunoglobulin of yolk (IgY). Different doses of VpAHPND IgY agent (0, 0.2% and 0.5%) were added in shrimp feeds and immunized the juvenile L. vannamei for 28 d. The growth rate and survival rate of the juveniles, the activity of immune enzymes and the relative expression level of immune genes in shrimp hepatopancreas were measured. The resistance to VpAHPND infection of immunized juveniles was determined by the immersion infection. The growth experiment results showed that after 28 d of immunization, there was no significant difference in the average growth rate, specific growth rate and survival rate of the juveniles between the immunized group and the control group. The immune function test results showed that after 14 d of immunization, compared with the control group, the 0.2% immunized group (0.2% IgY agent) gave significantly higher enzyme activities on phenoloxidase (PO), superoxide dismutase (SOD) and lysozyme in the hepatopancreas. The relative expression level of antibacterial peptide (Crustin) gene was also significantly increased, while the relative expression level of β-1,3-glucan binding protein-lipoprotein (β-GBP-HDL) gene was significantly reduced in the 0.2% IgY agent group. The results of the immersion infection showed that the survival rate of the juveniles in the 0.2% IgY agent group was significantly higher than that of the control group, and the relative percentage of survival (RPS) of 0.2% IgY agent was 63.77%. In conclusion, oral administration of VpAHPND IgY agent will not adversely affect the growth and survival of L. vannamei. The 0.2% IgY agent can significantly enhance the immune function of L. vannamei by increasing the enzyme activities of PO, SOD, LZM and the expression level of Crustin gene, thereby improving the ability of shrimps to resist VpAHPND infection. This study implied that VpAHPND IgY agent has a strong application potential for the prevention of AHPND.
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