文章摘要
大黄鱼肌纤维性状的全基因组关联分析
Genome-wide association study of muscle fiber related traits in large yellow croaker
投稿时间:2021-02-01  修订日期:2021-03-12
DOI:
中文关键词: 大黄鱼  肌纤维直径  肌纤维密度  ddRAD-seq  全基因组关联分析
英文关键词: Larimichthys crocea  muscle fibre diameter  muslce fibre density  ddRAD-seq  GWAS
基金项目:福建省科技重大专项专题项目(2020NZ08003);中央高校基本科研业务费专项资金资助(20720192010);厦门大学大学生创新创业训练计划项目(201910384050);福建省高校产学合作项目(2019N5001)
作者单位邮编
何骞 福建省海洋生物遗传育种重点实验室 361102
吴怡迪 福建省海洋生物遗传育种重点实验室 
周志雄 福建省海洋生物遗传育种重点实验室 
郑文彬 福建省海洋生物遗传育种重点实验室 
周祉吟 福建省海洋生物遗传育种重点实验室 
郝帅峰 福建省海洋生物遗传育种重点实验室 
魏煜麒 福建省海洋生物遗传育种重点实验室 
濮菲 福建省海洋生物遗传育种重点实验室 
陈佳 大黄鱼育种国家重点实验室 
徐鹏 福建省海洋生物遗传育种重点实验室 361102
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中文摘要:
      大黄鱼是我国近海重要经济鱼类,因养殖条件限制,其肉质难以满足消费者的需求。肌纤维组织学特征是鱼类肉质评价中的常用指标,肌纤维平均直径和密度对鱼类肉质有重要影响。为定位肉质相关遗传位点,解析大黄鱼肉质性状的遗传调控机制,并为养殖大黄鱼肉质选育提供基础,本研究首次对大黄鱼肌纤维性状进行全基因组关联分析。采集一个大黄鱼养殖群体的背部肌肉样本,通过组织切片获得了483尾大黄鱼的肌纤维直径及253尾的肌纤维密度数据。随后用双消化限制性酶切位点相关的DNA测序(ddRAD-seq)法进行基因分型,获得了25 520和25 309个高质量单核苷酸多态性(SNPs)位点用于全基因组关联分析(GWAS)。最终,在5条染色体上定位到了6个显著位点,并注释到了lpin2、adcyap1、btg1、tcp11l2、lifr、thbs4、gmeb1、slc2a1、myom1、myom2共10个候选基因。对这些候选基因的分析表明,大黄鱼的肉质与肌纤维组成和生长,以及能量代谢有关。lpin2、adcyap1、btg1等基因可能在决定大黄鱼肌肉的物理特性中发挥作用。本研究首次对大黄鱼肌纤维相关性状进行基因组定位,研究结果为大黄鱼分子标记辅助选育提供了重要的理论参考,并为后续开展针对养殖群体大黄鱼肉质性状的遗传改良提供了分子工具。
英文摘要:
      Large yellow croaker (Larimichthys crocea) is an important maricultured fish in China. In recent years, the meat quality of cultured large yellow croaker gradually decreases due to limited culture conditions. Histological characteristics of muscle fibers are commonly used index in meat quality evaluation, especially muscle fibre diameter (FD) and density (Fρ). To identify related SNPs and investigate the potential genetic architecture of meat quality, thereby providing basis for meat quality related selection. We conducted the first genome-wide association study (GWAS) on muscle fiber related traits of large yellow croaker. We collected the dorsal muscle of 500 cultured large yellow croaker and measured the FD and Fρ by using paraffin section and hematoxylin eosin (HE) stain. The collected samples were genotyped by ddRAD-seq (double-digest restriction-site associated DNA sequencing), and a total of 25 520 and 25 309 high quality SNPs were obtained for FD and Fρ respectively. GWAS analysis identified 6 significantly associated SNPs on 5 chromosomes, and ten candidate genes such as lpin2, adcyap1, btg1 and tcp11l2 were identified. These genes are involved in biological processes including muscle fiber growth and development, as well as energy metabolism. Our results indicate genes such as lpin2, lifr and btg1 may play roles in determine the physical characteristics of meat quality in large yellow croaker. This is the first study on genomic mapping of muscle fibre related traits in large yellow croaker. The results provide theoretical basis and potential molecular tools for subsequent genetic improvement of meat quality traits in cultured large yellow croaker.
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