文章摘要
低温对四川华鳊卵巢发育的影响及调控
The effects of low temperature on the ovarian development of Sinibrama taeniatus
投稿时间:2021-01-08  修订日期:2021-02-18
DOI:
中文关键词: 四川华鳊  低温  卵泡闭锁  生殖激素  凋亡和自噬
英文关键词: Sinibrama taeniatus  low temperature  follicular atresia  reproductive hormones  apoptotic and autophagy
基金项目:重庆市社会事业与民生保障科技创新专项(重点研发项目)(cstc2017shms-zdyf0201)
作者单位邮编
刘楠楠 西南大学生命科学学院 400715
程新凯 西南大学生命科学学院 
杨洋 西南大学生命科学学院 
刘娟 西南大学生命科学学院 
辜浩然 西南大学生命科学学院 
王志坚 西南大学生命科学学院 400715
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中文摘要:
      四川华鳊(Sinibrama taeniatus)是长江上游特有的小型经济鱼类,在野外其一年可以繁殖两次,但在26℃条件下进行人工养殖时其繁殖周期仅为14天,其中低温是限制其在野外繁殖的重要因素。为探究低温对四川华鳊卵巢发育的影响及调控方式,本研究对其产卵后雌鱼进行11℃的低温处理(LT组),并与26℃条件下(OT组)卵巢发育状况进行对比。通过形态学和组织学观察发现LT组卵巢发育被抑制,发生退化,卵母细胞明显发育不良,出现典型闭锁卵泡的特征。对雌鱼血清中部分生殖激素及卵巢中相应受体基因的表达水平进行测定,发现低温使FSH和DHP水平显著下降,并严重抑制激素受体基因fshr,lhcgr,pgr,esr1,ar的表达。通过转录组测序和分析发现,在低温处理过程中类固醇激素合成,细胞生长增殖以及凋亡和自噬相关通路被显著富集,与低温的影响显著相关。低温下调了类固醇激素合成和卵母细胞发育相关基因StAR,cyp17a1,hsd3b,cyp19a2,hsd17b1,vtg1,zp4,mmp9,mmp15以及细胞生长增殖和抗凋亡相关基因ccni,cdk16,igf1r,egfr,nobox,bcl2的表达,上调了促凋亡基因bax,tnf,tp53的表达。上述结果表明,低温导致卵泡闭锁的原因一方面可能是低温抑制生殖激素的合成和受体基因的表达,使卵巢发育和卵母细胞生长增殖受限;另一方面,可能是低温促使卵泡发生自噬凋亡,最终导致卵泡发生退化闭锁。
英文摘要:
      Sinibrama taeniatus is a kind of rare and small economical fish which distribute in the upper Yangtze River. S. taeniatus can reproduce twice a year in the wild, while the reproductive cycle of S. taeniatus reared under the temperature of 26℃ is only 14 days. Low temperature is a major factor limiting the reproduction of fishes in the wild, especially in winter. We found that the low temperature of 11℃significantly inhibits the ovarian development of S. taeniatus. To explore the effects of low temperature on ovarian development of S. taeniatus, the female fish after spawning were treated with a low temperature of 11℃ (LT groups), and the fish reared under the temperature of 26℃ (OT groups) severed as control groups. We compared the ovarian development of the LT groups and OT groups. The morphological and histological results showed that the ovarian development was inhibited in the LT groups and that the oocytes were underdeveloped and degenerated. Besides, the atretic follicles were observed in the ovaries of the LT groups. The results of enzyme-linked immunosorbent assay and RT-qPCR showed that the secretion of FSH and DHP, and the gene expression of fshr, lhcgr, pgr, esr1, and ar were significantly inhibited by the low temperature. Transcriptome sequencing results showed that the differential expressed genes were significantly enriched in steroid hormone synthesis, cell growth and proliferation, apoptosis, and autophagy pathways, indicating these pathways were affected by the low temperature. The expression of genes involved in pro-apoptotic (i.e. bax, tnf, and tp53) was up-regulated in the LT groups. However, the expression of genes involved in steroid hormone synthesis, oocyte development, cell growth and proliferation, and anti-apoptosis viz., StAR, cyp17a1, hsd3b, cyp19a2, hsd17b1, vtg1, zp4, mmp9, mmp15, ccni, cdk16, igf1r, egfr, nobox, and bcl2 were significantly down-regulated in the LT groups. It suggested that the low temperature may inhibit oocyte growth and proliferation, and promote autophagy and apoptosis of follicles which resulted in follicular atresia.
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