文章摘要
仿刺参幼参对亮氨酸最适需求量的研究
Optimum dietary leucine requirement of juvenile sea cucumber Apostichopus japonicas Selenka
投稿时间:2021-01-04  修订日期:2021-02-19
DOI:
中文关键词: 仿刺参  亮氨酸  生长  免疫  消化酶
英文关键词: Apostichopus  japonicas, leucine, growth, immunity, digestive  enzyme
基金项目:烟台市科技计划(2021YT06000221);山东省现代农业产业技术体系-刺参创新团队(SDAIT-22-06)
作者单位邮编
刘财礼 上海海洋大学 201306
王际英 山东省海洋资源与环境研究院 264006
李宝山 山东省海洋资源与环境研究院 
沈钰博 上海海洋大学 
孙永智 山东省海洋资源与环境研究院 
黄炳山 山东省海洋资源与环境研究院 
王世信 山东省海洋资源与环境研究院 
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中文摘要:
      为探究仿刺参幼参对亮氨酸的最适需求量,在基础饲料中分别添加0、0.80%、1.60%、2.40%、3.20%和4.00%的包膜亮氨酸,配成亮氨酸含量分别为1.29%(D1,对照组)、1.63%(D2)、1.98%(D3)、2.22%(D4)、2.58%(D5)和2.97%(D6)的6组实验饲料,饲喂初始体质量为(16.40±0.14)g的仿刺参幼参60 d。结果显示:①随饲料亮氨酸含量从1.29%提高到1.98%,仿刺参幼参的增重率和特定生长率显著升高,在D3组达到最大值100.84%,随亮氨酸含量进一步提高,增重率和特定生长率显著降低,D3、D4和D5组增重率和特定生长率显著高于对照组; ②随饲料亮氨酸含量从1.29%提高到1.98%,仿刺参体壁的粗脂肪含量显著升高,在D3组达到最大值5.50%,且显著高于其他组,随亮氨酸含量进一步提高,仿刺参体壁粗脂肪含量显著降低,各组间水分、粗蛋白和粗灰分含量均无显著性差异;随饲料亮氨酸含量的升高,仿刺参体壁亮氨酸和蛋氨酸含量显著提高;③随饲料亮氨酸含量从1.29%提高到1.98%,仿刺参肠道脂肪酶和蛋白酶活性显著提高,饲料亮氨酸含量进一步提高时,脂肪酶和蛋白酶活性均显著降低,D3组脂肪酶活性显著高于其他组,D2、D3和D4组蛋白酶活性显著高于其他组;④随饲料亮氨酸含量从1.29%提高到1.98%,仿刺参肠道谷草转氨酶、谷丙转氨酶活性和总抗氧化能力显著升高,超过1.98%后,谷草转氨酶活性趋于平稳,而谷丙转氨酶活性和总抗氧化能力显著降低,D3组谷丙转氨酶活性显著高于其他组,D3组总抗氧化能力显著高于D1、D2、D5和D6组,随饲料亮氨酸含量从1.29%提高到1.98%,仿刺参肠道丙二醛含量显著降低,当饲料亮氨酸含量超过2.22%后,丙二醛含量显著升高,D3和D4组丙二醛含量显著低于其他组。研究表明,以增重率为评价指标,经一元二次回归分析得出,体质量为16.40 g的仿刺参幼参对饲料中亮氨酸的最适需求量为2.11%(10.37%饲料蛋白)。
英文摘要:
      A 60-d feeding trial was conducted to determine the dietary leucine requirement of juvenile sea cucumber Apostichopus japonicus with initial body weight (16.40±0.14) g. Six experimental diets were formulated with the graded leucine levels 1.29% (D1, control group), 1.63% (D2), 1.98% (D3), 2.22% (D4), 2.58% (D5) and 2.97% (D6) dry diets. The results showed that: There were no differences on survival rate among all groups (96.00%~98.67%). Both weight growth rate (WGR) and the specific growth rate (SGR) increased with increasing leucine content until reaching peak levels at 1.98% dietary leucine, but decreased thereafter. The WGR and SGR of D3, D4 and D5 groups were significantly higher than D1 group. The WGR reached maximum value of 100.84% in the D3 group. There were no significant effects on ratio of intestine weight to body wall weight (IBR) and ratio of intestine length to body length (IBL) of sea cucumber. The crude lipid contents of body wall was increased when dietary leucine content increased from 1.29% to 1.98% but decreased when the dietary leucine content exceeded 1.98%, D3 group was significantly higher than other groups, but there were no significant effects on moisture, crude protein and crude ash contents. Both methionine and leucine content of body wall were significantly increased by dietary leucine, but there were no significant effects on total amino acids (TAA). Both lipase and protease activities of intestinal were increased with increasing levels of leucine up to 1.98% diet and then decreased. The lipase of D3 group was significantly higher than other groups, meanwhile, the protease of D2, D3 and D4 groups were significantly higher than other groups. All of alanine aminotransferase (ALT), asparate aminotransferase (AST) and total antioxidant capacity (T-AOC) activities of intestinal were increased when dietary leucine content increased from 1.29% to 1.98%. There were no significant effects on AST activity when the dietary leucine content exceeded 1.98%, but the ALT and T-AOC activities were decreased. The T-AOC activity of D3 group was significantly higher than D1, D2, D5 and D6 groups. The catalase (CAT) and superoxide dismutase (SOD) activities reached maximum value when the dietary leucine content was 2.22%. The CAT activity of D4 was significantly higher than D3, D5 and D6 groups, The SOD activity of D4 was significantly higher than D1, D2 and D6 groups. The malondialdehyde (MDA) content was decreased when dietary leucine content increased from 1.29% to 1.98% but increased when the dietary leucine content exceeded 2.22%, the MDA contents of D3 and D4 groups were significantly lower than other groups. With WGR as evaluation indicator, quadratic regression analysis showed that the optimum dietary leucine of sea cucumber with body weight 16.40 g was 2.11% diet (10.37% dietary protein).
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