文章摘要
黄曲霉毒素B1对黄颡鱼幼鱼生长及肝脏功能的影响
Effects of aflatoxin B1 on growth and liver function of juvenile P. fulvidraco
投稿时间:2020-12-30  修订日期:2021-02-07
DOI:
中文关键词: 黄曲霉毒素  黄颡鱼  生长  肝功能
英文关键词: aflatoxin , Pelteobagrus fulvidraco, growth, liver function.
基金项目:国家特色淡水鱼产业技术体系专项(CARS-46);省部共建淡水鱼类发育生物学国家重点实验室开放课题基金(2020KF006);2019大北农科研合作项目。
作者单位邮编
钟蕾 湖南农业大学 410128
王子琴 湖南农业大学 
王金龙 湖南省水产科学研究所 
夏立秋 湖南师范大学 
陈丽媛 江苏奥迈生物科技有限公司 
周建成 武汉大北农水产科技有限公司 
胡毅 湖南农业大学 410128
摘要点击次数: 81
全文下载次数: 0
中文摘要:
      为研究黄曲霉毒素对黄颡鱼(Pelteobagrus fulvidraco)幼鱼生长、肠道消化酶及肝脏功能的影响,用添加不同梯度黄曲霉毒素B1(AFB1)(0、50、100、200 μg/kg)的4种等氮等脂配合饲料饲喂初始体重为(6.00±0.10 g)的黄颡鱼幼鱼8周。实验结果表明:饲料中添加AFB1对黄颡鱼幼鱼的存活率、饲料系数和特定生长率均无显著影响(P>0.05),但使肠道胰蛋白酶活力显著提高(P < 0.05),而高含量AFB1(100 μg/kg和200 μg/kg)显著降低肠道淀粉酶和脂肪酶活力(P < 0.05);随着AFB1添加量的上升,血清中谷丙转氨酶、谷草转氨酶活性及葡萄糖、甘油三酯、总胆汁酸和总胆固醇含量显著升高(P < 0.05),肝脏中谷丙转氨酶、谷草转氨酶活性显著下降(P < 0.05);AFB1添加组的肝脏过氧化氢酶活力和丙二醛含量显著高于对照组(P<0.05),100 μg/kg 和200 μg/kg AFB1组超氧化物歧化酶活力显著高于对照组,其余各组无显著差异;饲料中添加AFB1显著上调了肝脏sod和cat基因及炎性因子il-1β、il-10和il-8基因的相对表达(P<0.05);通过组织学观察,AFB1会引起部分肝细胞出现轻微萎缩、肝细胞核移位、细胞界限模糊和肝细胞内空泡化的现象。综上所述,饲料中添加200 μg/kg AFB1对黄颡鱼幼鱼生长无影响,但50 μg/kg AFB1会影响肠道的消化吸收功能,同时引起肝脏氧化应激及炎性反应,造成肝脏功能损伤,因此,黄颡鱼幼鱼饲料中AFB1的浓度应不超过50 μg/kg。
英文摘要:
      Feed ingredients or products can breed mold and produce aflatoxin(AFT) contamination due to improper management of processing, transportation and storage in a hot and humid environment. AFT is the secondary metabolites of aspergillus flavus and aspergillus parasiticus, its derivatives are about 20 kinds. Aflatoxin B1(AFB1) is the most toxic and most carcinogenic in AFT. After being ingested by aquatic animals, AFB1 can be absorbed by the gastrointestinal tract quickly and enter the circulatory system, deposite in various tissues of the body. Meanwhile, AFB1 can convert into AFB1-8,9- epoxides, form adducts with binding to DNA, RNA and proteins in the form of covalent bonds, and damage hepatocytes. Related studies have shown that few studies on the toxicity mechanism of AFB1 to aquatic animals were discovered, the sensitivity of different aquatic animals to AFB1 was different and the resistance of warm-water fish to AFB1 was higher than that of cold-water fish. What's more, there were no detailed reports on the effect of AFB1 on P. fulvidraco. Therefore, an 8-week experiment was conducted to find out the tolerance and safe concentration of P. fulvidraco to aflatoxin and provide a basis for healthy farming. In this study, four experimental diets contained 0 (control), 50, 100, 200 μg/kg aflatoxin were prepared and the the growth performance, digestive enzyme activities in intestinal tract, biochemical indexes in hemolymph, liver tissue structure, antioxidant indexes in liver, liver inflammatory related gene expression of juvenile P. fulvidraco were measured. The results showed that: (1)There were no significant differences in survival rate, feed coefficient ratio and specific growth rates of juvenile P. fulvidraco among groups (P>0.05). The activity of trypsin in the AFB1 group significantly increased compared with the control group(P<0.05). When the AFB1 concentration reached 100 μg/kg, the amylase and lipase activities significantly decreased (P<0.05); (2)With increasing AFB1 concentration, the levels of aspatate transaminase(AST), alanine transaminase(ALT), glucose, triglyceride, total bile acid and total cholesterol contents in serum significantly decreased (P<0.05), and the AST, ALT activities in the liver significantly decreased (P<0.05); (3)With increasing AFB1 concentration, the catalase activity and malonaldehyde content in the liver significantly increased(P<0.05). The superoxide dismutase activity significantly increased when the AFB1 concentration reaches 100 μg/kg (P<0.05); (4)With increasing AFB1 concentration, the expression levels of sod, cat, il-1β, il-10 and il-8 in the liver were significantly up-regulated (P<0.05); (5)Histological observations showed that an increase in the concentration of AFB1 caused some liver cells to appear slight atrophy, hepatocyte nucleus shifted, cell boundary blurred, liver cell vacuolation degree increased. To sum up, there were no significant effect on the growth of juvenile P. fulvidraco in 200 μg/kg group, but 50 μg/kg AFB1 could affect the digestion and absorption function of the intestines, cause oxidative stress and inflammatory reactions in the liver, and result in liver function damage. This research could provide a basis for healthy breeding of juvenile P. fulvidraco, but the toxic mechanism of AFB1 to aquatic animals and the metabolic mechanism of AFB1 in aquatic animals need further research.
HTML     下载PDF阅读器
关闭

手机扫一扫看