文章摘要
黑斑蛙蝌蚪出血病病原菌的分离鉴定及药物敏感性分析
Isolation, identification and drug sensitivity of the pathogen causing hemorrhagic disease of Rana nigromaculata tadpole
投稿时间:2020-12-29  修订日期:2021-02-06
DOI:
中文关键词: 黑斑蛙  蝌蚪  嗜水气单胞菌  病原鉴定  药物敏感性
英文关键词: Rana nigromaculata  tadpole  Aeromonas hydrophila  Pathogen identification  drug sensitivity
基金项目:国家自然科学基金面上项目(32073020);湖南省教育厅科学研究重点项目(20A238);国家级大学生创新训练计划项目(s202010537005)
作者单位邮编
钟为铭 湖南农业大学 410128
彭芳 湖南农业大学 
陈康勇 上海海洋大学 
赵天宇 湖南农业大学 
邹钧 上海海洋大学 
高志鹏 湖南农业大学 410128
摘要点击次数: 54
全文下载次数: 0
中文摘要:
      为确定黑斑蛙蝌蚪出血病的病原菌并筛选出防治该病的药物,本研究从患出血病病蝌蚪体内分离出菌株KD-CXB-1,健康蝌蚪在人工感染该菌后出现了与自然感染蝌蚪相似的出血症状,同时从人工感染死亡蝌蚪体内分离到了与菌株KD-CXB-1形态特征及生理生化特性一致的优势菌株,以上结果表明KD-CXB-1为黑斑蛙蝌蚪出血病的病原菌。进一步,我们对菌株KD-CXB-1开展了形态学观察、革兰氏染色、生理生化指标测定、16S rDNA基因序列测定、系统发育树构建、药物敏感性测定。形态学观察显示该菌为杆状的革兰氏阴性菌,长度约1 μm,表面粗糙。通过16S rDNA基因序列比对、系统发育树构建、生理生化实验确定KD-CXB-1为嗜水气单胞菌。药物敏感性实验结果表明,KD-CXB-1对氟苯尼考、丁香、苏木等10种药物高度敏感;对乌梅、五倍子、黄芩3种药物中度敏感,对阿莫西林、大黄、青霉素等8种药物不敏感。本研究表明,嗜水气单胞菌是引起黑斑蛙蝌蚪出血病的病原菌,筛选出的10种高敏感药物为有效防治蝌蚪出血病提供了理论参考和实践依据。
英文摘要:
      To uncover the pathogen causing hemorrhagic disease of Rana nigromaculata tadpoles and screen out sensitive drugs to control the disease, in this study, a strain KD-CXB-1 was isolated from diseased tadpoles, healthy tadpoles showed hemorrhagic symptoms similar to those of naturally infected after being artificially infected with strain KD-CXB-1. Meanwhile, a dominant strain consistent with the morphological, physiological and biochemical characteristics of strain KD-CXB-1 was isolated from the dead tadpoles artificially infected. These results indicated that strain KD-CXB-1 was the pathogen causing hemorrhagic disease of R. nigromaculata tadpoles. Moreover, focused on strain KD-CXB-1, several experiments were performed including morphological observation, gram staining, physiological and biochemical tests, 16S rDNA gene sequencing, construction of phylogenetic trees, and drug susceptibility. The morphological observation showed that the KD-CXB-1 was a rod-shaped Gram-negative bacterium with the length of about 1 μm and rough surface. Strain KD-CXB-1 was identified as Aeromonas hydrophila by 16S rDNA sequencing and physiological and biochemical tests. Drug susceptibility tests indicated that strain KD-CXB-1 was highly sensitive to 10 drugs such as florfenicol, Syzygium aromaticum and Caesalpinia sappan; moderately sensitive to Prunus mume, Rhus chinensis and Scutellaria baicalensis, and insensitive to 8 drugs such as amoxicillin, Rheum palmatum and penicillin. In conclusion, our study proved that A. hydrophila was the pathogen causing hemorrhagic disease of R. nigromaculata tadpoles, and the highly sensitive drugs screened out by drug susceptibility tests may provide theoretical reference and practical basis for the effective control of hemorrhagic disease of R. nigromaculata tadpoles.
HTML     下载PDF阅读器
关闭

手机扫一扫看