文章摘要
黄连素对高脂诱导的斑马鱼肝脏细胞脂质代谢的影响
Effects of Berberine on Lipid Metabolism of Zebrafish Liver Cells Induced by High Fat
投稿时间:2020-12-25  修订日期:2021-02-13
DOI:
中文关键词: 高脂、黄连素、斑马鱼肝脏细胞、脂质代谢组学
英文关键词: high fat  berberine  zebrafish liver cells  lipid metabolomics
基金项目:国家自然科学基金(31472292)
作者单位邮编
刘瑾 上海交通大学农业与生物学院 200240
张美娟 上海交通大学农业与生物学院 
于成兵 上海交通大学农业与生物学院 
杨烁烁 上海交通大学农业与生物学院 
张京 上海交通大学农业与生物学院 
徐健雄 上海交通大学农业与生物学院 
徐维娜 上海交通大学农业与生物学院 200240
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中文摘要:
      为研究黄连素对高脂诱导的斑马鱼肝脏细胞脂质代谢组学的影响,以斑马鱼细胞为实验材料,通过棕榈酸钠(0.25mM,24h)诱导肝脏细胞脂质沉积模型,经黄连素(25μM,6h)处理后收集细胞。采用高效液相色谱-质谱联用技术进行脂质代谢组学分析,对不同处理的斑马鱼肝脏细胞的脂质代谢物进行筛选鉴定并对脂代谢相关因子的基因表达进行检测。结果显示,与对照组相比,高脂组共筛选出1761个差异离子,其中鉴定出147种差异代谢物,123个升高、24个降低;高脂+黄连素组共筛选出2728个差异离子,其中鉴定出346种差异代谢物,139个升高、207个降低。与高脂组相比,高脂+黄连素组共筛选出1605个差异离子,其中鉴定出259种差异代谢物,45个升高、204个降低。进一步对不饱和脂肪酸代谢通路研究发现,差异代谢产物二高-γ-亚麻酸(DGLA)和二高-α-亚麻酸(ETA)含量在高脂组中显著上升(P < 0.05),且添加了黄连素后含量显著下降(P < 0.05);与对照组组相比,高脂+黄连素组的DGLA和ETA含量并无显著差异。通过检测DGLA和ETA分解相关酶的基因(ATGL、HSL)表达量发现,与对照组相比,高脂组两者均显著(P<0.05)降低,添加了黄连素后均显著(P<0.05)升高;检测合成相关酶基因(ACC、Elovl6、Elovl7a、SCDb、FAS、FADS2)的表达量,结果显示,与对照组相比,高脂组组细胞内ACC、Elovl6和SCDb的表达量显著(P<0.05)升高,FADS2表达量显著(P<0.05)降低,而添加了黄连素后细胞内FADS2的表达量显著(P<0.05)升高,FAS、Elovl6、SCDb表达量显著(P<0.05)降低。综上所述,高脂诱导通过抑制脂质分解相关基因表达,促进脂质合成相关基因的表达进而引起脂肪在细胞中的过度沉积,添加适量黄连素可以显著改善这一现象。
英文摘要:
      In order to explore the effect of berberine on the lipid metabonomics of zebrafish liver cells induced by high fat, zebrafish cells were used as experimental materials, and sodium palmitate (0.25mM, 24h) was used to induce liver cell lipid deposition models. After treatment with berberine (25μM, 6h), the cells were collected. Using high performance liquid chromatography-mass spectrometry technology for lipid metabolomics analysis, the lipid metabolites of different treatments of zebrafish liver cells were screened and identified, and the gene expression of lipid metabolism-related factors was detected. The results showed that compared with the control group, a total of 1761 differential ions were screened in the high-fat group, among which 147 differential metabolites were identified, 123 were increased and 24 were decreased; the high-fat + berberine group screened a total of 2728 differences Ions, among which 346 different metabolites were identified, 139 increased and 207 decreased. Compared with the high-fat group, the high-fat + berberine group screened a total of 1605 differential ions, of which 259 differential metabolites were identified, 45 increased and 204 decreased. Further studies on the metabolic pathways of unsaturated fatty acids found that the content of the differential metabolites dihomo-γ-linolenic acid (DGLA) and dihomo-α-linolenic acid (ETA) increased significantly in the high-fat group (P <0.05), and added The content of berberine decreased significantly (P <0.05); compared with the control group, the content of DGLA and ETA in the high-fat+berberine group did not differ significantly. By detecting the expression of genes (ATGL, HSL) related to DGLA and ETA decomposition enzymes, it was found that compared with the control group, both of the high-fat group were significantly (P<0.05) reduced, and both were significantly (P<0.05) after the addition of berberine ) Increased; detection of the expression levels of synthesis-related enzyme genes (ACC, Elovl6, Elovl7a, SCDb, FAS, FADS2), the results showed that compared with the control group, the high-fat group intracellular ACC, Elovl6 and SCDb expression were significant (P<0.05) increased, FADS2 expression decreased significantly (P<0.05), and FADS2 expression in cells increased significantly (P<0.05) after berberine was added, FAS, Elovl6, and SCDb expressions were significantly increased (P <0.05) Decrease. To sum up, high-fat induction inhibits the expression of lipid-decomposition-related genes and promotes the expression of lipid-synthesis-related genes, thereby causing excessive deposition of fat in cells. Adding appropriate amounts of berberine can significantly improve this phenomenon.
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