文章摘要
饲料不同钙磷比对三疣梭子蟹幼蟹生长性能、矿物质沉积和能量代谢的影响
Influence of dietary calcium to phosphorus ratio on growth, minerals bioaccumulation and energe metabolism in juvenile swimming crab (Portunus trituberculatus)
投稿时间:2020-12-14  修订日期:2021-01-29
DOI:
中文关键词: 钙磷比  三疣梭子蟹  生长性能  血清生化指标  矿物质沉积  能量代谢
英文关键词: calcium to phosphorus ratio  Portunus trituberculatus  growth performance  hemolymph biochemical indicators  minerals bioaccumulation  energetic metabolism
基金项目:国家自然科学基金(32072987);浙江省自然科学基金项目(Y21C190016);国家虾蟹产业技术体系(CARS-48);国家重点研发计划(2018YFD0900400);国家海洋局海洋经济创新发展示范工作产业链协同创新类项目(NBHY-2017-S2);浙江省重大项目(2018C02037);浙江省水产动物营养与饲料技术服务团队(ZJANFTST2017-2);宁波大学王宽诚基金.
作者单位邮编
赵明明 宁波大学 315211
袁 野 宁波大学 
金 敏 宁波大学 
罗嘉翔 宁波大学 
王学习 宁波大学 
胡潇颖 宁波大学 
程 鑫 宁波大学 
时 博 宁波大学 
焦乐飞 宁波大学 
周歧存 宁波大学 315211
摘要点击次数: 120
全文下载次数: 0
中文摘要:
      本实验旨在研究饲料中不同钙磷比对三疣梭子蟹幼蟹生长性能、血清生化、矿物质沉积和能量代谢的影响。配制5种等氮等脂(46%粗蛋白和7%粗脂肪)钙磷比分别为1.00:2.00、1.00:1.50、1.00:1.00、1.00:0.75和1.00:0.50的实验饲料。选择初始体质量为(12.82±0.37)g的梭子蟹150只,随机分成5组(每组3个重复,每个重复10只),进行为期8周的养殖实验。结果表明:钙磷比为1.00:1.00和1.00:0.75的饲料,其特定生长率(SGR)和饲料效率(FE)显著高于其他组。钙磷比为1.00:0.50饲料组幼蟹血淋巴的谷草转氨酶(GOT)显著高于其他组,碱性磷酸酶(AKP)和谷丙转氨酶(GPT)活性随着饲料钙磷比的增加呈上升的趋势。幼蟹肝胰腺、肌肉和甲壳中的磷含量随着钙磷比的升高呈下降的趋势,且钙磷比1.00:2.00组幼蟹磷含量显著高于1.00:0.50组。钙磷比1.00:1.50和1.00:1.00组幼蟹肝胰腺ATP含量显著高于其他饲料组。幼蟹肝胰腺己糖激酶(HK)和琥珀酸脱氢酶(SDH)的活性随饲料钙磷比的升高呈先升高后下降的趋势,当钙磷比为1时,梭子蟹肝胰腺的HK和SDH酶活性最高。此外,钙磷比1.00:1.00组显著上调了梭子蟹肝胰腺电子传递链相关基因(nd1、sdhc、cytb和coxⅡ)的表达水平,当钙磷比为1.00:0.75时,显著上调了幼蟹肝胰腺线粒体能量代谢相关基因 (ATPase6、sirt3和nrf1)的表达量。综上所述,本实验条件下,以增重率为评价指标,通过回归模型得到梭子蟹最适钙磷比为1.06~1.26,在此范围内能促进三疣梭子蟹幼蟹的生长、维持组织的最适磷沉积以及很好地促进能量的产生。
英文摘要:
      An 8-weeks feeding trial was conducted to investigate the effects of dietary calcium/phosphorus ratios on the growth performance, minerals bioaccumulation, and energy metabolism in juvenile swimming crab (Portunus trituberculatus). Five semi-purified experimental diets were formulated to contain different ratios of calcium to phosphorus (1.00:2.00、1.00:1.50、1.00:1.00、1.00:0.75 and 1.00:0.50, respectively). A total of 150 swimming crab juveniles (initial weight: 12.82 ± 0.37 g) were randomly allocated into five groups with three replicates, each consisted of 30 crabs. The results indicated that crabs fed the diets with calcium to phosphorus ratio of 1.00:1.00 and 1.00:0.75 had significantly higher specific growth rate (SGR) and feed efficiency (FE) than those fed the other diets. The highest activity of GOT in hemolymph was observed at crabs fed the diet with calcium to phosphorus ratio of 1.00:0.50, AKP and GPT exhibited an increasing trend with the increase of dietary calcium to phosphorus ratios. Moreover, the content of phosphorus in tissues significantly increased as dietary calcium to phosphorus ratios decreasing from 1.00:0.50 to 1.00:2.00. Furthermore, the highest ATP content in hepatopancreas was observed in crab fed the diets calcium/phosphorus ratios of 1.00:1.50 and 1.00:1.00. The activities of enzymes such as hexokinase (HK) and succinate dehydrogenase (SDH) which related to energy metabolism were significantly higher in hepatopancreas of crabs fed the diet with calcium to phosphorus ratio of 1.00:1.00. Crabs fed the diet containing calcium to phosphorus ratio 1.00:1.00 also showed significantly higher expression of genes involved in electron transport chain complex, such as nd1, sdhc, cytb and coxII than those fed the other diets. The genes related to mitochondrial energetic metabolisms, such as atpase6, sirt3, and nrf1 were significantly up-regulated in crabs fed the diet with calcium and phosphorus ratio of 1.00:0.75. Overall, based on broken-line and quadratic regression analysis between PWG against dietary calcium to phosphorus ratios, the optimal dietary calcium to phosphorus ratio was estimated to be 1.06 - 1.26, and were recommended to maintain optimal phosphorus bioaccumulation and promote energy production for juvenile swimming crab.
HTML     下载PDF阅读器
关闭

手机扫一扫看