文章摘要
长丰鲢生长性状相关微卫星标记的初步筛选
Preliminary screening of microsatellite DNA markers associated with growth traits in Changfeng silver carp (Hypophthalmichthys molitrix)
投稿时间:2020-12-13  修订日期:2021-02-04
DOI:
中文关键词: 长丰鲢  微卫星标记  生长性状  相关分析
英文关键词: Changfeng silver carp  microsatellite marker  growth trait  correlation analysis
基金项目:现代农业产业技术体系建设专项资金(CARS-45-01); 国家淡水水产种质资源库项目
作者单位邮编
吴新燕 中国水产科学研究院长江水产研究所 430223
梁宏伟 中国水产科学研究院长江水产研究所 
罗相忠 中国水产科学研究院长江水产研究所 
王丹 中国水产科学研究院长江水产研究所 
沙航 中国水产科学研究院长江水产研究所 
邹桂伟 中国水产科学研究院长江水产研究所 430223
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中文摘要:
      为了筛选出与长丰鲢生长性状相关的分子标记,以指导其种质鉴定和选择育种工作,本研究利用15个多态微卫星标记,对6、17和36月龄长丰鲢进行了遗传多样性分析和生长性状关联分析。结果显示:各月龄长丰鲢的观测杂合度(Ho)和期望杂合度(He)均高于0.5,PIC均值分别为0.555、0.445和0.490,表明各月龄长丰鲢群体均具有较高的遗传多样性。其中,染色体LG15上的位点BL55在3个月龄组均含有231 bp片段的等位基因,该位点上的基因型225/231、231/245分别对长丰鲢起负面影响和正面影响;为了研究该位点在其他鲢群体中的适用性,分别选取长江、湘江2个野生的鲢群体进行验证。研究表明,231 bp片段的等位基因在长江鲢群体和湘江鲢群体中均存在,据此初步确定标记BL55为与鲢生长性状相关的候选标记,对鲢的遗传改良和选择育种均有一定的参考价值。
英文摘要:
      Changfeng silver carp (Hypophthalmichthys molitrix) is a new silver carp bred by the Yangtze River Fisheries Research Institute of the Chinese Academy of Fishery Sciences. It has many advantages, such as a quick growth rate, a high body size, and a neat appearance, which are associated with more excellent growth characteristics than those of common H. molitrix. Up to the present, Changfeng H. molitrix has been spread in 26 provinces (cities and areas) in China, and exceptional economic and social benefits have been achieved. However, the study on H. molitrix at home and abroad mainly focuses on developing microsatellite markers, analyzing genetic structure, and establishing the genetic linkage map. Few molecular markers associated with H. molitrix growth traits have been thought of, and it is not known whether these markers can be used in the selection and breeding of Changfeng H. molitrix. This study aimed to screen out the molecular markers related to the growth traits of Changfeng H. molitrix to guide the germplasm identification and selective breeding. In this study, 15 polymorphic microsatellite markers were used to analyze the genetic diversity and growth trait association of Changfeng H. molitrix at 6, 17 and 36 months. The results show that the observed heterozygosity (Ho) and expected heterozygosity (He) of each month-old group changfeng H. molitrix were higher than 0.5. The average PIC values were 0.555, 0.445, and 0.490 respectively at each age; indicating that the population was highly genetically diverse. The results of associations between microsatellite markers and growth traits revealed that locus BL55 and BL109 were significantly associated with body height at 6-month-old (P<0.05); S65 was found Significantly associated with body height at 17-month-old (P<0.05); locus BL55 was significantly associated with total length (P<0.05), and BL106-2 and BL116 were significantly associated with body weight at 36-month-old (P<0.05). The different growth traits associated with different months of age of the same marker may be due to the different growth trends of Changfeng H. molitrix. Locus BL55 on chromosome 15 has the same allele containing DNA fragment length of 231 at different month ages, and it is preliminarily speculated that the genotypes 225/231, as well as 231/245, will have negative and positive effects, respectively. To further examine whether this locus can be used for molecular marker-assisted breeding in H. molitrix populations, two wild populations of H. molitrix from Yangtze River and Xiangjiang were selected for analysis. The results showed that the allele of the 231bp fragment existed in both the Yangtze River and Xiangjiang populations. This result preliminarily confirmed that the marker BL55 was determined as a candidate marker related to growth traits, which has a specific reference value for genetic improvement and selective breeding of H. molitrix.
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