文章摘要
弓背青鳉早期胚胎色素细胞发生及相关基因表达规律分析
The development of pigment cells and analysis of the expression of related genes in the early embryo of Oryzias curvinotus
投稿时间:2020-11-09  修订日期:2021-03-06
DOI:
中文关键词: 弓背青鳉  色素细胞发生  早期胚胎  群体分化
英文关键词: Oryzias curvinotus  pigment cells  embryo  genetic differentiation
基金项目:国家自然科学(41806195,31972794);2019年度创新强校工程项目(230419069,230419055);省部共建淡水鱼类发育生物学国家重点实验室开放课题(2020KF004);[Supported by National Natural Science Foundation of China (No.41806195, 31972794);
作者单位邮编
黄嘉慧 广东海洋大学水产学院 524088
郭昱嵩 广东海洋大学水产学院 
杜娟 广东海洋大学水产学院 
董忠典 广东海洋大学水产学院 
王中铎 广东海洋大学水产学院 524088
摘要点击次数: 95
全文下载次数: 0
中文摘要:
      体表色素具有保护鱼类免受紫外线辐照伤害等重要功能。依照自然选择理论,不同群体的体色形成过程可能受到光照等环境因素的选择作用而发生遗传分化。为了检验这一假说,本研究以相同条件下人工繁育的不同纬度的弓背青鳉(Oryzias curvinotus)群体(饶平、高桥、三亚)后代(F6)为材料,使用荧光倒置显微镜对早期胚胎的黑色素细胞和虹彩色素细胞进行观察统计,并利用实时荧光定量PCR(qRT-PCR)分析4种色素细胞发生关键基因(黑色素形成的限速酶基因tyr、虹彩色素细胞相关基因alk、sox10和pax3)在高桥群体后代早期发育(6-10,12,14肌节期)过程中的表达加以验证。结果表明,高纬度饶平群体早期胚胎的黑色素细胞和虹彩色素细胞分别集中出现于8肌节期和10肌节期,占比为81.9%和52.1%;相应地,高桥群体早期胚胎的黑色素细胞和虹彩色素细胞分别集中出现于7和11肌节期,占比47.7%和42.1%;而三亚群体早期胚胎的黑色素细胞和虹彩色素细胞分别集中出现于8和12肌节期,占比 44.0%和62.2%。对高桥F6代胚胎样本的色素细胞发生相关基因qRT-PCR检测确定:tyr表达水平从6肌节期开始上调,至10肌节期趋于平稳;而抑制黑色素形成通路并启动虹彩细胞分化通路的sox10和pax3基因与下游的虹彩色素细胞相关基因alk在10-14肌节期均呈现一致的单峰表达模式。综上,本研究支持弓背青鳉早期发育阶段的黑色素细胞和虹彩色素发生模式与鱼类经典理论相符,受tyr、alk、sox10、pax3等基因的有序表达的调控,且证实了发生阶段存在由遗传因素决定的群体分化,低纬度群体的虹彩细胞发生更晚,其具体机制仍待进一步研究。
英文摘要:
      The pigments have lots of functions such as protecting fish from radiation and changing their body color for courtship. According to the theory of natural selection, the process of formation of pigments of different groups may genetically differentiated due to the selection effect of environment light and so on. Oryzias curvinotus has strong fertility, short generation cycle, early sensitivity to water quality and environmental changes, and wide adaptability to salinity. They used to live in the layer water, which is widely distributed in Guangdong coastal areas and is expected to be developed as a model species for monitoring coastal water environment in China. To test the above hypothesis, in this study, different latitudes groups (Raoping, Gaoqiao, Sanya) of O. curvinotus were bred under the same conditions, which their progenies (F6) were used as materials. The iridophores and melanophores of embryo were observed by fluorescence inverted microscope, in addition, the expression of four main genes (tyr、alk、sox10、pax3), in the early development of the offspring of Gaoqiao population was analyzed by real-time fluorescence quantitative PCR, and the observation results were verified. The results showed that from south to north: the melanophores and iridophores of the progenies of Raoping population were concentrated in 8 and 10 somites stage, accounting for 81.9% and 52.1% respectively; Accordingly, the population of Gaoqiao was concentrated in 7 and 11 somites stage, accounting for 47.7% and 42.1% respectively; And Sanya was concentrated in 8 and 12 somites stage, accounting for 44% and 62.2%. RT-qPCR analysis of Gaoqiao embryo confirmed that the expression of tyr, the rate limiting enzyme of melanin formation, was up-regulated from the 6 to 10 somites, and tended to be stable; And the sox10 and pax3 genes, which inhibit the formation pathway of melanin and initiate the differentiation of iridescent cells, showed the single peak expression pattern from the 10 to 14 somites stage as the downstream alk genes. In conclusion, through this study, we can confirm that the melanophores and iridophores in the early developmental stage of O. curvinotus obeyed the classic pathway of fish, and is regulated by the orderly regulation of genes such as tyr, alk, sox10, pax3, and the iridescent cells in the southern population generally occur later, which supports the existence of population genetic differentiation, but the specific mechanism remains to be studied.
HTML     下载PDF阅读器
关闭

手机扫一扫看