| （Objective）The aim of this study was to investigate the effects of kelp enzymatic extract on the growth and hepatopancreas transcriptome of channel catfish (Ictalurus punctatus). (Method) We prepared different diets with enzymatic extract of kelp addition amount of 0.3 g/kg (KP3), 0.5 g/kg (KP5), 1.0 g/kg (KP10), 1.5 g/kg (KP15), 2.0 g/kg (KP20) to feed channel catfish for 60 days.Channel catfish [initial body weight (51.18±1.14)g] were randomly divided into 6 groups with 3 replicates per group and 40 fishes per replicate.. (Results) The results showed that: 1) Compared with the control group S, the addition of kelp enzymatic extract in diet has no significant effect on the SR of channel catfish, the SGR increased by 1.20% to 5.39%, and the FCR decreased by 1.65 % to 9.09%; A quadratic polynomial regression analysis was carried out with SGR and FCR as the targets respectively., and the optimal addition amount of kelp enzymatic hydrolysis extract in the feed was 0.98 g/kg and 0.93 g/kg; The moisture, crude fat, crude protein and ash of fish have no significantly difference in all groups. 2) Height of duplicature in KP3，height and width of duplicature and thickness of muscularis in KP5，thickness of muscularis in KP10 were all significantly higher than those in group S. 3) Total RNA was extracted from S and KP10 groups’ hepatopancreas. Transcriptome sequencing results showed that there were 81 significantly down-regulated genes and 199 up-regulated genes; GO functional classification analysis results indicated that, DEGs are annotated to items such as DNA transcription, metal ion binding, membrane and membrane composition; According to the results of KEGG pathway analysis, we found that DEGs are mainly enriched in 12 related pathways including cell proliferation and differentiation, hormone regulation, lipid metabolism, carbohydrate metabolism, growth factor metabolism and so on. (Conclusion) Our Studies have indicated that adding kelp enzymatic extract to diet can promote the growth of channel catfish, reduce feed coefficient, improve intestinal morphology, and strengthen the glucose and lipid metabolism of liver and pancreas，the suitable addition amount was 0.93 g / kg ~ 0.98 g / kg.