文章摘要
日粮海带酶解提取物对斑点叉尾鮰生长和肝胰脏转录组表达的影响
Effects of Enzymatic Extract of Kelp on the Growth and hepatopancreas transcriptome of channel catfish (Ictalurus punctatus)
投稿时间:2020-10-09  修订日期:2021-01-19
DOI:
中文关键词: 海带酶解提取物  生长  肝胰脏转录组  斑点叉尾鮰
英文关键词: kelp enzymatic extract  growth  liver transcriptome  channel catfish
基金项目:海带酶解提取物对斑点叉尾鮰生长的影响与氧化豆油损伤修复研究
作者单位邮编
吕昊 苏州大学基础医学与生物科学学院 215123
叶元土 苏州大学基础医学与生物科学学院 215123
易皓明 苏州大学基础医学与生物科学学院 
孙飞 苏州大学基础医学与生物科学学院 
石瑶瑶 苏州大学基础医学与生物科学学院 
吕斌 苏州大学基础医学与生物科学学院 
周伟 山东荣成鸿德海洋生物科技有限公司 
蒋蓉 无锡三智生物科技有限公司 
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中文摘要:
      (目的)为了研究饲料中海带酶解提取物对斑点叉尾鮰生长和肝胰脏转录组的影响,(方法)在日粮中分别添加0.3 g/kg (KP3)、0.5 g/kg (KP5)、1.0 g/kg (KP10)、1.5 g/kg (KP15)、2.0 g/kg(KP20)的海带酶解提取物,以初始体重为(51.18±1.14)g的斑点叉尾鮰为试验对象,随机分为6组,每组3个重复,每个重复40尾鱼,在池塘网箱中养殖60 d。(结果)结果显示:1)与对照(S)组相比,饲料添加海带酶解提取物对斑点叉尾鮰的存活率(SR)没有显著影响,特定生长率(SGR)提高了1.20%~5.39%,饲料系数(FCR)降低了1.65%~9.09%;分别以SGR和FCR为目标进行二次多项式回归分析,得到海带酶解提取物在饲料中的最适添加量分别为0.98 g/kg和0.93 g/kg;KP10~KP20组肥满度(CF)和脏体比(VSI)显著降低,KP10组肝体比(HIS)显著降低;各组全鱼的水分、粗脂肪、粗蛋白质和粗灰分含量差异不显著。2)KP3组肠道皱襞高度,KP5组肠道皱襞高度、宽度、肌层厚度,KP10组的皱襞宽度均显著高于S组。3)选取S组与KP10组鱼肝胰脏提取总RNA后进行转录组测序和基因差异表达分析,表达显著下调的基因数有81个,显著上调的有199个;GO功能分类分析结果显示,DEGs被注释到DNA转录、金属离子结合、膜及膜组成成分等条目上;KEGG pathway分析结果显示,DEGs主要富集到细胞增殖与分化、激素调控、脂类代谢、糖类代谢以及生长因子代谢等相关的12个显著差异代谢通路。(结论)研究表明,日粮添加海带酶解提取物可以促进斑点叉尾鮰生长,降低饲料系数,改善肠道形态结构,加强肝胰脏的糖脂代谢能力,适宜添加量为0.93 g/kg~0.98 g/kg。
英文摘要:
      (Objective)The aim of this study was to investigate the effects of kelp enzymatic extract on the growth and hepatopancreas transcriptome of channel catfish (Ictalurus punctatus). (Method) We prepared different diets with enzymatic extract of kelp addition amount of 0.3 g/kg (KP3), 0.5 g/kg (KP5), 1.0 g/kg (KP10), 1.5 g/kg (KP15), 2.0 g/kg (KP20) to feed channel catfish for 60 days.Channel catfish [initial body weight (51.18±1.14)g] were randomly divided into 6 groups with 3 replicates per group and 40 fishes per replicate.. (Results) The results showed that: 1) Compared with the control group S, the addition of kelp enzymatic extract in diet has no significant effect on the SR of channel catfish, the SGR increased by 1.20% to 5.39%, and the FCR decreased by 1.65 % to 9.09%; A quadratic polynomial regression analysis was carried out with SGR and FCR as the targets respectively., and the optimal addition amount of kelp enzymatic hydrolysis extract in the feed was 0.98 g/kg and 0.93 g/kg; The moisture, crude fat, crude protein and ash of fish have no significantly difference in all groups. 2) Height of duplicature in KP3,height and width of duplicature and thickness of muscularis in KP5,thickness of muscularis in KP10 were all significantly higher than those in group S. 3) Total RNA was extracted from S and KP10 groups’ hepatopancreas. Transcriptome sequencing results showed that there were 81 significantly down-regulated genes and 199 up-regulated genes; GO functional classification analysis results indicated that, DEGs are annotated to items such as DNA transcription, metal ion binding, membrane and membrane composition; According to the results of KEGG pathway analysis, we found that DEGs are mainly enriched in 12 related pathways including cell proliferation and differentiation, hormone regulation, lipid metabolism, carbohydrate metabolism, growth factor metabolism and so on. (Conclusion) Our Studies have indicated that adding kelp enzymatic extract to diet can promote the growth of channel catfish, reduce feed coefficient, improve intestinal morphology, and strengthen the glucose and lipid metabolism of liver and pancreas,the suitable addition amount was 0.93 g / kg ~ 0.98 g / kg.
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