文章摘要
酵母水解物对低盐胁迫凡纳滨对虾非特异性免疫及抗氧化能力的影响
Effect of Yeast Hydrolysate on Non-specific Immunity and Antioxidant Ability of Litopenaeus vannamei under Low Salinity Stress
投稿时间:2020-09-26  修订日期:2020-11-06
DOI:
中文关键词: 凡纳滨对虾  酵母水解物  低盐度胁迫  非特异性免疫  抗氧化能力  
英文关键词: Litopenaeus vannamei  yeast hydrolysate  low salinity stress  non-specific immunity  antioxidant capacity
基金项目:国家重点研发计划(NO. 2019YFD0900200)资助
作者单位邮编
陈永康 广东海洋大学 水产学院 524088
陈泽恩 广东海洋大学 水产学院 
梁武辉 广东海洋大学 水产学院 
黎国勇 广东海洋大学 水产学院 
胡骏鹏 安琪酵母股份有限公司 
杨志龙 安琪酵母股份有限公司 
聂琴 安琪酵母股份有限公司 
迟淑艳 广东海洋大学 水产学院 524088
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中文摘要:
      本实验旨在研究饲料中添加酵母水解物对凡纳滨对虾在低盐度胁迫条件下非特异性免疫和抗氧化能力的影响。选取凡纳滨对虾[初始体质量(15.82±0.08)g],平均分为2组,对照组投喂基础饲料(Y0),实验组投喂添加了3%酵母水解物的实验饲料(Y3),在室内养殖15d后分别放入盐度为4‰(S4)和28‰(S28)的水体中进行盐度胁迫实验,每组6个重复,每个重复30尾虾(根据饲料和盐度不同组合,分组命名为Y0S4、Y0S28、Y0S3、Y0S28)。结果表明:饲料中添加3%酵母水解物对凡纳滨对虾血清酚氧化酶(PO)、总一氧化氮合成酶(TNOS)活性没有显著影响,但低盐度胁迫1h时,Y3S4组PO、TNOS活性显著高于Y0S4组;盐度因素对PO、TNOS活性产生显著影响,低盐度胁迫1h时,S28组PO、TNOS活性显著高于S4;饲料中添加3%酵母水解物显著提高了凡纳滨对虾肝胰腺超氧化物歧化酶(SOD)活性;盐度因素对酸性磷酸酶(ACP)活性产生了显著影响,S28组ACP活性显著高于S4。由此可见,在饲料中添加酵母水解物能够提高凡纳滨对虾肝胰腺SOD活性,提高对虾的抗氧化能力;在遭遇低盐度胁迫时,对虾能迅速恢复PO、TNOS活性至正常水平,从而提高凡纳滨对虾的抵抗不良环境的能力。
英文摘要:
      The purpose of this experiment is to study the effects of yeast hydrolysate on the non-specific immunity and antioxidant capacity of Litopenaeus vannamei under low salinity stress. Shrimps [initial weight 15.82±0.08) g] were randomly divided into 2 groups. The control group was fed with basic feed (Y0), and the experimental group was fed with experimental feed (Y3) supplemented with 3% yeast hydrolysate. After 15 days of raising, they were transferred to water of salinity 4‰ (S4) and salinity 28‰ (S28) for low salinity stress with 3 replicates tanks of each group and 30 individuals in each tank. Groups were named Y0S4、Y0S28、Y0S3、Y0S28 according to different combination of feed and salinity conditions. The results showed that the addition of yeast hydrolysate had no significant effects on the serum phenol oxidase (PO) and total nitric oxide synthase (TNOS) activities of L. vannamei. However, activities of PO and TNOS in Y3S4 group were significantly higher than those in Y0S4 group at 1h; salinity factor had a significant impact on the activities of PO and TNOS and the activities of PO and TNOS in S28 group were significantly higher than S4 at 1h. Yeast hydrolysate significantly increased the activities of superoxide dismutase (SOD) in the hepatopancreas of L. vannamei; salinity factor had a significant effect on ACP activities, and ACP activity in S28 group was significantly higher than S4. These results showed that yeast hydrolysate can increase the SOD activity under normal conditions and increase PO and TNOS activities under salinity stress, thereby improving the resistance to low salinity stress.
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