文章摘要
湖栖鳍虾虎鱼皮肤和眼睛转录组比较分析
Comparative analysis of skin and eye transcriptome in the Gobiopterus lacustris
投稿时间:2020-09-13  修订日期:2020-10-28
DOI:
中文关键词: 湖栖鳍虾虎鱼  皮肤  眼睛  RNA-Seq  色素形成  体色
英文关键词: G. lacustris  skin  eye  RNA-Seq  pigment formation  body color
基金项目:国家自然科学基金(41806195, 31201996),广东省科技厅项目(2017A030303075),广东海洋大学创新强校项目(230419069,230419055),广东海洋大学“南海学者计划”青年人才项目(QNXZ201903, 201807),广东海洋大学博士启动项目
作者单位邮编
黎学友 广东海洋大学水产学院 524088
黄承勤 广东海洋大学水产学院 
谢明花 广东海洋大学水产学院 
汪淳 广东海洋大学水产学院 
王中铎 广东海洋大学水产学院 
郭昱嵩 广东海洋大学水产学院 
董忠典 广东海洋大学水产学院 524088
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中文摘要:
      湖栖鳍虾虎鱼是一种对盐度适应性强、易于实验室饲养的小型鱼类。其体表几乎透明,肉眼可见机体组织器官,是理想的模式生物和环境指示物种。为探究湖栖鳍虾虎鱼体色透明的成因,本研究对湖栖鳍虾虎鱼的皮肤和眼睛进行了转录组比较分析。测序原始数据经de novo拼接、组装共获得103 686个单基因(unigene),N50和平均长度分别为1 456和2 490 bp。在NR、NT、SwissProt、PFAM、KOG、GO及KEGG数据库中对上述unigenes进行注释,分别有57 380、37 343、51 277、47 020、47 555和25 604个unigenes获得注释。对差异基因进行KEGG富集发现,在黑色素生成途径中,tyr、tyrp1和tyrp2等基因在皮肤中明显下调。另外基因表达差异分析显示,在湖栖鳍虾虎鱼皮肤和眼睛中共有8 113个差异表达基因(DEGs),其中3 174个DEGs在皮肤中上调,4 939个DEGs在眼睛中上调。通过实时荧光定量PCR(qPCR)对10个DEGs的验证,确定了RNA-Seq分析正确。本研究丰富了湖栖鳍虾虎鱼的体色研究,为湖栖鳍虾虎鱼遗传资源的利用提供了数据,也为进一步研究鱼类绚丽的体色形成机制提供了参考。
英文摘要:
      Gobiopterus lacustris is a kind of small fish with strong adaptability to salinity and easy to raise in laboratory. It is an ideal model organism and environmental indicator species, whose body is transparent and its organs can be seen through naked eyes. In order to explore the cause of the transparent body color of G. lacustris, the transcriptome analysis of skin and eye of G. lacustris was carried out. A total of 103 686 unigenes were obtained by de novo splicing. The N50 and average length were 1 456 and 2 490 bp, respectively. In NR, NT, Swissprot, Pfam, KOG, GO and KEGG databases, 57 380, 37 343, 51 277, 47 020, 47 555 and 25 604 unigenes were annotated respectively. KEGG enrichment showed that tyr, tyrp1 and tyrp2 were significantly down regulated in melanin production pathway. In addition, the results showed that there were 8 113 differentially expressed genes (DEGs) in the skin and eyes of G. lacustris, of which 3 174 were up-regulated in the skin and 4 939 in the eyes. The verification of 10 DEGs by qPCR confirmed that the RNA-Seq analysis was correct. This study enriches the body color research of G. lacustris, provides data for the utilization of G. lacustris genetic resources, and also provides a reference for further study on the formation mechanism of gorgeous body color of fish.
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