| The shrimp postlarva vitrified syndrome broke out in the spring of 2020 and spread explosively along the coastal areas from south to north of China. In this study, the pathogen was isolated and identified, and the histopathology was investigated. The postlarva symptoms included emaciation, dark cloud, decreased activity, anorexia, empty intestinal tract and stomach. The hepatopancreatic was atrophy, blurring of contour, paleness and even vitrified syndrome. Histopathological analysis showed that the epithelial cells of liver tubule were necrotic and exfoliated, liver tubule filled with a large amount of debris tissue, and leaving continuous glassy homogeneous areas. The intestinal tract was filled with tissue fragments, chorionic membrane falls off and even disappears. Ultrastructural pathological examination showed that the membranes of epithelial cells were ablated, organelles disintegrated, and nuclei were solidified. Subsequently, the cells disintegrated, fell off, and even the hepatic tubular tissue structure was ablated. The bacteria were found in the hepatopancreatic, intestinal tract and gastric mucosa. The dominant strain was rod-shaped and curved, and no virions were found. Two dominant bacteria (Lv-A, Lv-B) were isolated from the diseased shrimp postlarva. Artificial infection experiments illustrated that the strains of Lv-A and Lv-B were the causative pathogens with a median lethal dose of 1.62×103 CFU/mL and 5.38×103 CFU/mL, respectively. Based on molecular analyses (16S rDNA and gyrB), Lv-A and Lv-B were high similar to Vibrio alginolyticus, Vibrio neocaledonicus and Vibrio parahaemolyticus, preliminarily named as Shrimp postlarva bacterial vitrified syndrome (BVS). The chemotherapeutant sensitivity tests illustrated that Lv-A and Lv-B was sensitive to minocycline, doxycycline, nalidixic acid, etc. and resistant to neomycin, pipemidic, rifampicin, etc. This study provides theoretical basis and technical support for the effective prevention of BVS and the healthy development of shrimp industry.