文章摘要
草鱼呼肠孤病毒II型自然发病与人工注射感染草鱼的病理症状和病毒分布研究
Studies on pathological symptoms and virus distribution in natural and artificial injection infection Ctenopharyngodon idella with grass carp reovirus type II
投稿时间:2020-09-08  修订日期:2021-02-05
DOI:
中文关键词: 草鱼  草鱼呼肠孤病毒  症状  组织病理  病毒分布
英文关键词: grass carp (Ctenopharyngodon idella)  grass carp reovirus (GCRV)  symptom  histopathology  virus distribution
基金项目:国家重点基础研究发展计划 (2018YFD0900504)
作者单位邮编
杨玲 华中农业大学水产学院 430070
苏建国 华中农业大学水产学院 430070
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中文摘要:
      草鱼呼肠孤病毒(GCRV)是危害草鱼健康的主要病原之一,引起严重的草鱼出血病。GCRV-II型是目前我国水产养殖中的流行毒株。目前自然发病和人工感染GCRV-II型病毒草鱼的临床症状、病理特征和病毒分布的区别尚不清楚。在本研究中,我们比较了自然发病和人工注射感染草鱼的临床症状,发现患病草鱼的眼眶、鳃盖、口腔、腹部、鳍条基部、肠道、肌肉出现明显的点状出血,且后者的出血情况比前者更为严重;比较组织病理切片,发现草鱼感染组织出现不同程度的充血和红细胞充盈现象,其中,人工注射感染草鱼的肠道、肌肉和肝胰脏的病变程度更为严重,呈现出较为严重的组织内出血和病变,而自然发病草鱼的鳃和脾脏的病变程度更为严重,鳃呈现出较为严重的充血和炎症增生物,脾脏出现大面积含铁血黄素沉积块病灶。qRT-PCR和Western Blot检测显示,GCRV在不同的组织中均有分布,头肾在两种感染方式的患病草鱼中的病毒量都比较高,人工感染时肝胰脏、肠道和肌肉的病毒量较高,自然发病时中肾、鳃和脾脏的病毒量较高。因此,在人工注射感染时,肝胰脏、肠道和肌肉可能是GCRV入侵的主要靶器官,而在自然发病时,鳃可能是GCRV入侵的主要靶器官,病毒聚集靶器官都是头肾,头肾是GCRV的“病毒库”。本研究有助于深入解析GCRV的发病机制和草鱼的免疫响应,也为草鱼出血病的防治和疫苗的研发提供参考。
英文摘要:
      Grass carp reovirus (GCRV) is one of the main pathogens that causes severe grass carp hemorrhagic disease. GCRV type II (GCRV-II) is currently prevalent strain in China. At present, the difference between the clinicopathological symptoms and virus distribution of naturally and artificially injection infected Ctenopharyngodon idella with GCRV-II virus is still unclear. In this study, we compared the clinical symptoms of naturally diseased and artificially injected Ctenopharyngodon idella, and found that the eye socket, operculum, mouth cavity, abdomen, fin base, intestine and muscle of the diseased grass carp showed obvious punctate hemorrhage. The bleeding of the latter was more serious than that of the former. Comparing histopathological sections, it was found that the infected tissues had different degrees of congestion and erythrocytes accumulation. Among them, the intestines, muscles and hepatopancreas of the artificial injection of Ctenopharyngodon idella were more severely affected, showing more serious intra-tissue bleeding and lesions. The gills and spleen of naturally diseased Ctenopharyngodon idella are more severely affected, the gills show more serious hyperaemia and inflammatory hyperplasia, and large areas of hemosiderin agglutination lesions in the spleen. qRT-PCR and Western blot tests showed that GCRV is distributed in different tissues. The head kidney has the relatively high viral load in diseased fish of the two infection methods. The viral load of the hepatopancreas, intestine and muscle is higher in artificially infected fish. The amount of virus is higher in the trunk kidney, gill and spleen in naturally diseased fish. Therefore, the hepatopancreas, intestine and muscle may be the main target organs of GCRV invasion in artificial infection, while the gill may be the main target organ of GCRV invasion in the case of natural disease, and the target organ for virus accumulation is the head kidney. The head kidney is the "virus library" of GCRV. This study is helpful to deeply analyze the pathogenesis of GCRV and the immune responses of grass carp, and also provides references for the prevention and treatment of grass carp haemorrhagic disease and the development of vaccine.
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