文章摘要
中华鲟DIGIRR与TRAF6基因的克隆与表达
Cloning and expression of DIGIRR and TRAF6 genes of Acipenser sinensis
投稿时间:2020-08-24  修订日期:2020-11-02
DOI:
中文关键词: 中华鲟  ASDIGIRR  ASTRAF6  Poly(I:C)  基因克隆与表达
英文关键词: Acipenser sinensis  ASDIGIRR  ASTRAF6  Poly(I:C)  Gene cloning and expression
基金项目:农业农村部淡水生物多样性保护重点实验室开放课题
作者单位邮编
张利凤 长江大学 434020
汤东东 长江大学 
郑楚文 长江大学 
邓丹 长江大学 
郭慧芝 长江大学 
张书环 贵州中医药大学 
张文兵 长江大学 
许巧情 长江大学 434025
李宁求 中国水产科学研究院珠江水产研究所 
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中文摘要:
      中华鲟是处在硬骨鱼类与软骨鱼类之间的中国古老的珍稀鱼类之一,但在人工繁殖和养殖过程中,容易受到不同疾病的危害,因此,需要深入研究中华鲟的免疫调控为其疾病预防提供理论依据。单个免疫球蛋白白细胞介素-1受体相关分子(single immunoglobulin interleukin-1 receptor related molecule,SIGIRR)和肿瘤坏死因子受体相关因子6(tumor necrosis factor receptor related factor 6,TRAF6)是toll样受体(Toll-like receptor,TLR)信号通路的2个重要的信号转导元件。本研究鉴定了中华鲟的SIGIRR和TRAF6的同源物,分别命名为ASDIGIRR(Acipenser sinensis DIGIRR)和ASTRAF6(Acipenser sinensis TRAF6),并研究它们在正常组织中的表达和Poly(I:C)诱导后的表达模式。结果显示,ASDIGIRR和ASTRAF6在健康鲟的10个组织中均有表达,且分别在肠道和头肾组织中表达量最高;用Poly(I:C)孵育中华鲟脾脏细胞后,ASDIGIRR在3 h时的表达量显著下调,在6 h时恢复至正常水平,后在24 h显著上调达到最高值,随后开始下调至48 h时仍显著高于对照组,而ASTRAF6在6 h达到最高表达量,维持到24 h后再开始下调,48 h下降至最低的水平。研究表明,ASDIGIRR和ASTRAF6在中华鲟抵御病原入侵的免疫防御过程中起着重要作用。
英文摘要:
      Acipenser sinensis is one of the ancient old and rare fish in China between teleost fish and cartilaginous fish, but it is vulnerable to different diseases in the process of artificial reproduction and culture, so it is necessary to deeply study the immune regulation of Acipenser sinensis to provide theoretical basis for its prevention of diseases. A single Ig-IL-1R-related molecule (SIGIRR) and tumor necrosis factor receptor-associated factor 6 (TRAF6) are two important signal transduction elements in the signal transduction pathway of toll-like receptors. In this study, homologues of SIGIRR and TRAF6 of Acipenser sinensis, named ASDIGIRR and ASTRAF6, were identified, and their expression levels in normal tissues and Poly (I:C) induced expression were investigated. Results show that ASDIGIRR and ASTRAF6 were expressed in 10 tissues of healthy Sturgeon, and the highest expression was found in intestinal tract and head kidney, respectively. After incubation with Poly (I:C), the expression of ASDIGIRR was significantly down-regulated at 3 h, and returned to the normal level at 6 h, then increased to the highest level at 24 h and decreased at 48 h but higher than the control group. While the expression of ASTRAF6 reached the highest level at 6 h, and decreased to the lowest level at 48 h after 24 h. The results show that ASDIGIRR and ASTRAF6 play an important role in the immune defense of Acipenser sinensis against pathogen invasion.
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