文章摘要
饲料中胆碱含量对大口黑鲈幼鱼生长性能、体成分和血清抗氧化机能的影响
Effect of dietary choline levels on growth performance, body composition and serum antioxidant activity of juvenile largemouth bass, Micropterus salmoides
投稿时间:2020-08-20  修订日期:2020-09-21
DOI:
中文关键词: 大口黑鲈  氯化胆碱  生长性能  脂肪含量  抗氧化能力  溶菌酶活性  谷草转氨酶活性
英文关键词: Largemouth bass  Choline chloride  Growth performance  Lipid content  Antioxidant capacity  Lysozyme activity  Aspartate aminotransferase activity
基金项目:农业部现代农业产业技术体系专项( CARS-46)
作者单位邮编
周 越 上海海洋大学 201306
王伟隆 上海海洋大学 
李松林 上海海洋大学 
吕红雨 上海海洋大学 
陈乃松 上海海洋大学 
张 松 广州市联鲲生物科技有限公司 
黄旭雄 上海海洋大学 201306
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中文摘要:
      为研究饲料中胆碱含量对大口黑鲈幼鱼生长性能、体成分以及血清抗氧化机能的影响,在基础饲料中分别添加0(对照组)、700、1 400、2 100和2 800 mg kg-1 mg/kg的氯化胆碱,配制成5组等氮等能的实验饲料(胆碱实测含量分别为2 369.57、2 716.90、2 993.49、3 443.60和3 799.05 mg/kg饲料),分别投喂初始体质量为 (20.00±0.10) g的大口黑鲈幼鱼56 d。结果显示,实验鱼的增重率(WGR)和特定生长率(SGR)随着饲料中氯化胆碱添加量的提高呈先升后降,在添加量为2 100 mg/kg饲料组均达到最大值,显著高于对照组(P<0.05) ;饲料中添加氯化胆碱对实验鱼的存活率(SR)、肝体比(HSI)、脏体比(VSI)和肥满度(CF)无显著影响。当氯化胆碱添加量达到2 100 mg/kg饲料及以上时,鱼体肌肉和肝脏的粗脂肪含量均显著低于对照组。相比于对照组,添加1 400- ~2 800 mg/kg饲料的氯化胆碱组鱼体血清中总抗氧化能力(T-AOC)、超氧化歧化酶(SOD)活性和过氧化氢酶(CAT)活性显著提高,血清中丙二醛(MDA)的含量显著降低;氯化胆碱添加量为2 100 mg/kg饲料组鱼体血清溶菌酶(LZM)和谷草转氨酶(AST)的活性分别达到最大值和最小值,且与对照组差异显著。研究表明,饲料中适量添加氯化胆碱可显著提高大口黑鲈幼鱼的生长性能、降低肝脏脂肪含量及增强血清抗氧化能力。回归分析显示大口黑鲈幼鱼饲料中氯化胆碱建议添加量为2 008.50 mg/kg~2 398.16 mg/kg (饲料胆碱含量为3 432.09 mg/kg~3 530.23 mg/kg)。
英文摘要:
      An experiment was conducted to investigate the effects of dietary choline content on growth performance, body composition, and serum antioxidant activity of juvenile largemouth bass, Micropterus salmoides. Graded choline chloride levels of 0,700,1400,2100 and 2800 mg/kg were supplemented to basal diet to formulate fiver isonitrogen and isoenergy practical diets containing choline of 2 369.57(control group), 2 716.90, 2 993.49, 3 443.60 and 3 799.05 mg/kg, respectively. Each diet was randomly assigned to triplicate cage of 20 fish with an initial average weight of (20.00±0.10) g for 56 days. The results showed that the weight gain rate (WGWGR) and specific growth rate (SGR) of the fish first increased and then decreased with the increase of dietary choline chloride, and reached the maximum when the dietary supplementation was 2 100 mg/kg, which were significantly higher than those of the control group (P<0.05). The survival rate (SR), hepatopancreas somatic index (HSI), viscera somatic index (VSI), and condition factor (CF) of the fish were not significantly affected by dietary choline chloride supplementation (P>0.05). When the dietary choline chloride supplementation reached 2 100 mg/kg or above, the lipid contents in muscle and liver of the fish were significantly decreased than those in control (P<0.05). Compared with the control group, the total antioxidant capacity, superoxide dismutase and catalase activities were significantly increased and the content of malondialdehyde in serum was significantly decreased in the groups which were dietary supplemented 1 400 mg/kg, 2 100 mg/kg and 2 800 mg/kg choline chloride (P<0.05). The maximum of lysozyme activity and minimum of aspartate aminotransferase activity appeared in the group treated with dietary choline chloride of 2 100 mg/kg the dosage was, which were significantly different from those of the control group (P<0.05). All these results indicated that the suitable dietary choline chloride supplementation could significantly improve growth performance, reduce liver fat content, and enhance the serum's antioxidant capacity. Regression analysis showed that the recommended amount of choline chloride in the practical diet of juvenile largemouth bass was 2 008.50 mg/kg~2 398.16 mg/kg (the dietary choline content was 3 432.09 mg/kg~3 530.23 mg/kg).
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