文章摘要
美洲鳗鲡致病性鲍曼不动杆菌的分离、鉴定及致病性分析
Isolation, identification, and pathogenicity analysis of Acinetobacter baumannii in Anguilla rostrate
投稿时间:2020-08-19  修订日期:2020-09-10
DOI:
中文关键词: 美洲鳗鲡  鲍曼不动杆菌  分离鉴定  16S rDNA  药敏
英文关键词: Anguilla rostrata  Acinetobacter baumannii  isolation and identification  16S rDNA  antibiontic sensitivity test
基金项目:海南省自然科学基金(2019RC078);海南省重大科技计划项目(ZDKJ2019011);海南省南海名家青年项目
作者单位邮编
孙云 海南大学 570228
何明旺 海南大学 
张盼盼 海南大学 
李海平 海南大学 
曹贞洁 海南大学 
周永灿 海南大学 570228
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中文摘要:
      为确定海南某鳗鲡养殖场患病美洲鳗鲡大量死亡的原因,从患病美洲鳗鲡肝脏和脾脏中分离获得1株优势菌株AB01,回归感染证实该菌株为导致此次美洲鳗鲡患病的病原菌。经生理生化鉴定、16S rDNA基因序列比对及系统发育分析,判定菌株AB01为鲍曼不动杆菌(Acinetobacter baumannii)。致病性分析表明,菌株AB01对美洲鳗鲡的半致死浓度为4.63×106 CFU/mL。组织病理学观察显示,患病美洲鳗鲡的肝脏、脾脏均发生不同程度的病变,其中,肝脏出现部分肝索排列紊乱,细胞肿胀,胞核溶解,胞质疏松,结构模糊,有大量大小不等的空泡等;脾脏中胞质多处稀疏,伴有大面积损伤等。药敏检测表明,鲍曼不动杆菌AB01对头孢噻吩、头孢唑啉、阿奇霉素、丁胺卡那霉素、利福平、依诺沙星、环丙沙星、氧氟沙星敏感,对氟苯尼考、氨苄西林等9种抗生素中度敏感,对氨曲南和四环素等20种抗生素多重耐药性。研究结果为美洲鳗鲡的疫病防控提供理论依据。
英文摘要:
      In order to determine the cause of the large number of deaths of the sick American eel (Anguilla rostrate) in an aquaculture fish farm in Haikou (Hainan), one dominant strain (named AB01) was isolated from the liver and spleen of the diseased American eels. The dominant strain AB01 was identified as Acinetobacter baumannii based on the results form physiological and biochemical testing, sequence alignment of 16S rDNA gene and phylogenetic analysis. The challenge test in healthy American eels by artificial reentry indicated that A. baumannii was the pathogenic bacteria. Further analysis of pathogenicity showed that the semi-lethal concentration of A. baumannii (AB01) was 4.63×106 CFU/mL. Histopathological observation of liver from diseased Anguilla anguilla showed the arrangement of some hepatic cords was disordered, and the cells were swollen, together with dissolved nucleus, loose cytoplasm, and fuzzy structure. Moreover, a lot of vacuoles of different sizes were present in liver. In addition, spleen from diseased Anguilla rostrata presented sparse cytoplasm and extensive injury. The results of antimicrobial susceptibility test showed that A. baumannii (AB01) was resistant to many antibiotics, but was highly sensitive to cephalothin, cefazolin, azithromycin, amikacin, enoxacin, rifampicin, ciprofloxacin, ofloxacin, and medium sensitivity to nine antibiotics, such as florfenicol and so on. Moreover, A. baumannii (AB01) was resistant to twenty antibiotics, such as aztreonam and so on. Thus, this study provides a scientific support for the prevention and control of the disease of the American eel.
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