文章摘要
高温胁迫下大黄鱼肝脏的蛋白组学
Proteome of liver tissue of large yellow croaker(Larimichthys crocea)under high temperature stress
投稿时间:2020-08-16  修订日期:2020-09-16
DOI:
中文关键词: 大黄鱼  高温胁迫  肝脏  TMT  蛋白组学  PRM
英文关键词: Larimichthys crocea  high temperature  liver  TMT  proteomics  PRM
基金项目:国家重点研发计划(2018YFC1406302)和福建省自然科学基金(2019J01694)
作者单位邮编
章孝颖 集美大学水产学院农业农村部东海海水健康养殖重点实验室 361021
蔡晓芳 集美大学水产学院农业农村部东海海水健康养殖重点实验室 
刘贤德 集美大学水产学院农业农村部东海海水健康养殖重点实验室 
赵虹博 集美大学水产学院农业农村部东海海水健康养殖重点实验室 
宋普庆 自然资源部第三海洋研究所 
张静 集美大学水产学院农业农村部东海海水健康养殖重点实验室 361021
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中文摘要:
      为探究大黄鱼在高温胁迫条件下蛋白的表达情况,本研究应用TMT(tandem mass tag)标记结合液相色谱—串联质谱(LC-MS/MS)技术对大黄鱼耐高温组和不耐高温组的肝脏组织进行蛋白组学分析。共鉴定到3 369个蛋白,其中有687个差异表达蛋白,包括281个上调蛋白和406个下调蛋白。用PRM(parallel reaction monitoring, PRM)对随机挑选的13个蛋白进行验证,其结果与TMT标记的蛋白组学分析结果一致。随后,通过生物信息学分析,发现高温胁迫对大黄鱼的蛋白质折叠、能量代谢有显著影响,其中热休克蛋白70(heat shock proteins 70, HSP70)、钙网蛋白(calreticulin, CRT)、葡萄糖调节蛋白(glucose-regulated protein, GRP)和丙酮酸激酶(pyruvate kinase, PK)在大黄鱼应对高温胁迫时发挥着重要的作用。本研究有助于进一步解析大黄鱼高温适应机制,并为选育大黄鱼耐高温品种提供参考。
英文摘要:
      In order to explore the changes in protein expression of large yellow croaker under high temperature stress, tandem mass tag(TMT)combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to perform proteome analysis on the liver tissues between the high temperature tolerance group and the high temperature sensitive group of large yellow croaker. A total of 3 369 proteins were identified, of which 687 were differentially expressed proteins, including 281 up-regulated proteins and 406 down-regulated proteins. 13 randomly selected proteins were validated with parallel reaction monitoring (PRM) assays, which showed that they were consistent with the TMT-labeled proteome analysis results. Subsequently,through bioinformatics analysis and found that high temperature stress has a significant impact on the protein folding and energy metabolism of large yellow croaker. Among them, heat shock proteins 70, calreticulin, glucose-regulated protein and pyruvate kinase play an important role in the response to high temperature stress, This study is helpful to further analyze the high temperature adaptation mechanism of large yellow croaker, and provides reference for breeding high temperature resistant species of large yellow croaker.
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