文章摘要
鳗鲡疱疹病毒对欧洲鳗鲡的致病性研究
Pathogenicity of Anguillid herpesvirus to European eels, Anguilla anguilla
投稿时间:2020-08-09  修订日期:2020-09-16
DOI:
中文关键词: 欧洲鳗鲡  鳗鲡疱疹病毒  致病性  脱黏败血综合征  病原
英文关键词: Anguilla anguilla, Anguillid herpesvirus, pathogenicity, mucus sloughing and hemorrhagic septicemia disease, pathogen
基金项目:福建省公益类科研院所基本科研专项(2018R1019-5);国家自然科学基金项目(31101933);福建省农业科学院项目(A2016-3, AC2017-1)
作者单位邮编
陈强 福建省农业科学院生物技术研究所 350003
李英英 福建省农业科学院生物技术研究所 
杨金先 福建省农业科学院生物技术研究所 
宋铁英 福建省农业科学院生物技术研究所 
葛均青 福建省农业科学院生物技术研究所 350003
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中文摘要:
      为了明确鳗鲡疱疹病毒(Anguillid herpesvirus, AngHV)的致病性,用一株从鳗鲡“脱黏败血综合征”病料中分离的AngHV(NA16108),研究了其对欧洲鳗鲡幼鳗的致病性。结果显示,注射AngHV的鳗鲡体表出现黏液脱落、鳍条出血、红头等症状;鳃部出现黏液增多、出血,肝脏褪色、肿大,脾脏和肾脏肿大等病变;进一步的组织病理学观察发现,鳗鲡的体表黏液及黏膜上皮细胞脱落,次级鳃瓣增生、出血、呼吸细胞肿胀坏死,脾脏细胞坏死、黑色素细胞聚集,肾小管管壁上皮细胞坏死、玻璃样病变、管腔变窄等病理症状;这与鳗鲡“脱黏败血综合征”的发病特征是一致的。致死率分析显示,从第4天开始攻毒组鳗鲡开始出现死亡,第7天和第14天的累计死亡率分别达到26.7%和56.7%;荧光定量PCR检测显示,在攻毒鳗鲡肝脏、脾脏、肾脏、肠道、鳃和皮肤黏液中均可检测到AngHV;另外,用鳗鲡卵巢细胞系(eel ovary cell line, EO)从攻毒鳗鲡主要内脏器官中重新分离出AngHV;这表明鳗鲡发生了AngHV的系统侵染。本研究表明,AngHV是鳗鲡“脱黏败血综合征”的致病病原,这为深入开展该病的发病机制及防控研究奠定了重要基础。
英文摘要:
      In order to determine the pathogenicity of Anguillid herpesvirus (AngHV), an AngHV strain, NA16108, isolated from “mucus sloughing and hemorrhagic septicemia disease” samples was used to study its pathogenesis to juvenile European eel, Anguilla Anguilla. The results showed that the body surface of the AngHV-injected A. anguilla had the symptoms of mucus exfoliation, fin hemorrhages, red head, etc. The diseased A. anguilla had pathological changes of mucus increase and hemorrhages of gill, discoloration of liver and swelling of spleen and kidney, etc. Further histopathological analysis showed that the skin had exfoliation of mucus and mucosa flat epithelial cells, gill had secondary lamellar hyperplasia, blood vessel hemorrhages, swelling and necrosis of respiratory cells, spleen had necrosis and aggregation of melanocytes, and kidney had necrosis wall cells and narrowing lumen of renal tubular, which are consistent with the clinical characteristics of A. anguilla "mucus sloughing and hemorrhagic septicemia disease". The mortality analysis showed that AngHV infected A. anguilla began to die from the 4th day post injection, and the cumulative mortality on the 7th and 14th day reached up to 26.7% and 56.7%, respectively. Quantitative real-time PCR analysis showed that AngHV could be detected in the liver, spleen, kidney, intestine, gill and skin mucus of AngHV infected A. anguilla, and AngHV could be re-isolated by EO cell line from the main internal organs, which indicating that the A. anguilla had a systemic infection of AngHV. The results indicated that AngHV is the pathogenic agent of "mucus sloughing and hemorrhagic septicemia disease", which laid an important foundation for further study on the pathogenesis and prevention and control of the disease.
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